ArticleParasites & vectors2018
A new TaqMan method for the reliable diagnosis of Ehrlichia spp. in canine whole blood.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 37 citations in OpenAlex.
- A multidimensional analysis of the risk of infection with Ehrlichia canis among urban dogs in Iquitos, Peru.Scientific reports · 2025Article
- Visual Detection of Canine Monocytic Ehrlichiosis Using Polymerase Chain Reaction-Based Lateral Flow Biosensors.Animals : an open access journal from MDPI · 2025Article
- Developing diagnostic tools for canine periodontitis: combining molecular techniques and machine learning models.BMC veterinary research · 2023Article
- Pathogenic Rickettsia, Anaplasma, and Ehrlichia in Rhipicephalus microplus ticks collected from cattle and laboratory hatched tick larvae.PLoS neglected tropical diseases · 2023Article
- A community approach for pathogens and their arthropod vectors (ticks and fleas) in cats of sub-Saharan Africa.Parasites & vectors · 2022Article
- Systematic evaluation of TaqMan real-time polymerase chain reaction assays targeting theVeterinary world · 2022Article
- A community approach of pathogens and their arthropod vectors (ticks and fleas) in dogs of African Sub-Sahara.Parasites & vectors · 2021Article
- Survey of Ticks and Tick-Borne Rickettsial and Protozoan Pathogens in Eswatini.Pathogens (Basel, Switzerland) · 2021Article
- First Evidence ofPathogens (Basel, Switzerland) · 2021Article
- Comparative Analysis of Genome of Ehrlichia sp. HF, a Model Bacterium to Study Fatal Human Ehrlichiosis.BMC genomics · 2021Article
- Molecular Diagnosis, Prevalence and Importance of Zoonotic Vector-Borne Pathogens in Cuban Shelter Dogs-A Preliminary Study.Pathogens (Basel, Switzerland) · 2020Article
- Molecular screening of Anaplasmataceae in ticks collected from cattle in Corsica, France.Experimental & applied acarology · 2020Article
- Ehrlichia spp. and Anaplasma spp. in Xenarthra mammals from Brazil, with evidence of novel 'Candidatus Anaplasma spp.'Scientific reports · 2020Article
- Emergence of a NovelMicroorganisms · 2019Article
- Complete Genome Sequence of anMicrobiology resource announcements · 2019Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEhrlichiosis is an important emerging infectious disease of the canid family and humans worldwide. To date, no extensive evaluation or validation of a molecular diagnostic test for ehrlichiosis has been published. Here, we present data for a newly designed TaqMan assay and compare its performance to a commercial technology (PCRun®). Both of these real-time methods of analysis were evaluated using a comprehensive number of prospective and retrospective samples collected from dogs exhibiting symptoms of ehrlichiosis.
resultsWhole blood samples collected from dogs, retrospectively in the United Kingdom and prospectively in Israel, were analysed for the presence of Ehrlichia canis and Ehrlichia minasensis DNA using the TaqMan PCR, developed specifically for this study. The results were compared to those of a real time commercial isothermal amplification method (PCRun® system developed by Biogal Galed Labs ACS, Galed, Israel). The sensitivity and specificity (CI: 95%) of the TaqMan PCR and PCRun® were both determined to be 100% and absolute, for all of the samples tested. Interestingly, both tests were demonstrated to be highly comparable, irrespective of differences in amplification chemistry or sequences targeted. Host differences, incidence of disease and geographical location of the isolates had little impact on the positivity recorded by each of the diagnostic methods.
conclusionsIt was evident that both amplification methods were equally suited for diagnosing canine ehrlichiosis and while the PCRun® clearly amplified all clinically relevant Ehrlichia species known to infect dogs and humans, the TaqMan method was more specific for E. canis and E. minasensis. This work demonstrates that despite good analytical sensitivities and specificities for Ehrlichia spp. neither method could fully account for the clinical diagnosis of thrombocytopenia.
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