ArticleBiology of reproduction2018
DNA methylomes of bovine gametes and in vivo produced preimplantation embryos.
Article in Biology of reproduction, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed.
- Impact of Nutrition on Embryo Production in Cattle: Mechanistic Insights.Animals : an open access journal from MDPI · 2026Review
- Epigenetic Regulation of Production Traits in Ruminants: Implications for Breeding and Selection.Biology · 2026Review
- The bull sperm epigenome: role in male fertility and transmission to the offspring.The Journal of reproduction and development · 2026Review
- Effect of bovine oocyte transportation system on embryonic quality.The Journal of reproduction and development · 2025Article
- Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro.Reproduction (Cambridge, England) · 2025Article
- The effects of breeding young bulls and cowsAnimal reproduction · 2025Review
- The dominant follicle: the final frontier in bovine oocyte development.Animal reproduction · 2025Article
- Defining bovine CpG epigenetic diversity by analyzing RRBS data from sperm of Montbéliarde and Holstein bulls.Frontiers in cell and developmental biology · 2025Article
- Molecular factors driving the development of bovine embryos and embryo-like structures.Animal reproduction · 2025Review
- Review
- Strengths and limitations of reduced representation bisulfite sequencing (RRBS) in the perspective of DNA methylation analysis in fish: a case-study on rainbow trout spermatozoa.Fish physiology and biochemistry · 2024Article
- In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†.Biology of reproduction · 2024Article
- Pleomorphic adenoma gene1 in reproduction and implication for embryonic survival in cattle: a review.Journal of animal science · 2024Review
- TET enzyme driven epigenetic reprogramming in early embryos and its implication on long-term health.Frontiers in cell and developmental biology · 2024Review
- Single-cell RNA-seq and single-cell bisulfite-sequencing reveal insights into yak preimplantation embryogenesis.The Journal of biological chemistry · 2024Article
- Molecular and cellular programs underlying the development of bovine pre-implantation embryos.Reproduction, fertility, and development · 2023Article
- Maternal restricted- and over- feeding during gestation perturb offspring sperm epigenome in sheep.Reproduction (Cambridge, England) · 2023Article
- Establishment of bovine trophoblast stem cells.Cell reports · 2023Article
- Beneficial Effect of Polysaccharide Gel Made of Xanthan Gum and Locust Bean Gum on Bovine Oocytes.International journal of molecular sciences · 2023Article
- One-carbon metabolite supplementation to heifers for the first 14 d of the estrous cycle alters the plasma and hepatic one-carbon metabolite pool and methionine-folate cycle enzyme transcript abundance in a dose-dependent manner.Journal of animal science · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
DNA methylation is an important epigenetic modification that undergoes dynamic changes in mammalian embryogenesis, during which both parental genomes are reprogrammed. Despite the many immunostaining studies that have assessed global methylation, the gene-specific DNA methylation patterns in bovine preimplantation embryos are unknown. Using reduced representation bisulfite sequencing, we determined genome-scale DNA methylation of bovine sperm and individual in vivo developed oocytes and preimplantation embryos. We show that (1) the major wave of genome-wide demethylation was completed by the 8-cell stage; (2) promoter methylation was significantly and inversely correlated with gene expression at the 8-cell and blastocyst stages; (3) sperm and oocytes have numerous differentially methylated regions (DMRs)-DMRs specific for sperm were strongly enriched in long terminal repeats and rapidly lost methylation in embryos; while the oocyte-specific DMRs were more frequently localized in exons and CpG islands (CGIs) and demethylated gradually across cleavage stages; (4) DMRs were also found between in vivo and in vitro matured oocytes; and (5) differential methylation between bovine gametes was confirmed in some but not all known imprinted genes. Our data provide insights into the complex epigenetic reprogramming of bovine early embryos, which serve as an important model for human preimplantation development.
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Registered trials
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