ArticleJournal of visualized experiments : JoVE2018
The MPLEx Protocol for Multi-omic Analyses of Soil Samples.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Integrating multi-omics technologies to decipher microbiome functions.Nature communications · 2026Review
- Metaproteomics uncovers the functional capacity of a soil microbiome.Scientific reports · 2026Article
- Reduced legacy precipitation decreases microbial community growth efficiency and alters soil organic carbon in a California grassland.Microbiome · 2026Article
- Optimizing multiomics sample preparation: comparative evaluation of extraction protocols for HepG2 cells.Analytical and bioanalytical chemistry · 2026Article
- Decomposition causes short-term increases in functional molecular diversity of dissolved organic matter.Nature communications · 2025Article
- Untargeted, tandem mass spectrometry metaproteome of Columbia River sediments.Microbiology resource announcements · 2024Article
- Optimizing MS-Based Multi-Omics: Comparative Analysis of Protein, Metabolite, and Lipid Extraction Techniques.Metabolites · 2024Article
- Aromatic amino acid metabolism and active transport regulation are implicated in microbial persistence in fractured shale reservoirs.ISME communications · 2024Article
- Article
- The endohyphal microbiome: current progress and challenges for scaling down integrative multi-omic microbiome research.Microbiome · 2023Article
- Graphene oxide exposure alters gut microbial community composition and metabolism in an in vitro human model.NanoImpact · 2023Article
- Article
- Interactive effects of depth and differential irrigation on soil microbiome composition and functioning.Frontiers in microbiomes · 2023Article
- A Mineral-Doped Micromodel Platform Demonstrates Fungal Bridging of Carbon Hot Spots and Hyphal Transport of Mineral-Derived Nutrients.mSystems · 2022Article
- Genome-Resolved Metaproteomics Decodes the Microbial and Viral Contributions to Coupled Carbon and Nitrogen Cycling in River Sediments.mSystems · 2022Article
- Uncovering Hidden Members and Functions of the Soil Microbiome UsingJournal of proteome research · 2022Article
- Synthetic Soil Aggregates: Bioprinted Habitats for High-Throughput Microbial Metaphenomics.Microorganisms · 2022Article
- An optimized approach and inflation media for obtaining complimentary mass spectrometry-based omics data from human lung tissue.Frontiers in molecular biosciences · 2022Article
- Moisture modulates soil reservoirs of active DNA and RNA viruses.Communications biology · 2021Article
- Soil Metaproteomics for the Study of the Relationships Between Microorganisms and Plants: A Review of Extraction Protocols and Ecological Insights.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Mass spectrometry (MS)-based integrated metaproteomic, metabolomic, and lipidomic (multi-omic) studies are transforming our ability to understand and characterize microbial communities in environmental and biological systems. These measurements are even enabling enhanced analyses of complex soil microbial communities, which are the most complex microbial systems known to date. Multi-omic analyses, however, do have sample preparation challenges, since separate extractions are typically needed for each omic study, thereby greatly amplifying the preparation time and amount of sample required. To address this limitation, a 3-in-1 method for the simultaneous extraction of metabolites, proteins, and lipids (MPLEx) from the same soil sample was created by adapting a solvent-based approach. This MPLEx protocol has proven to be both simple and robust for many sample types, even when utilized for limited quantities of complex soil samples. The MPLEx method also greatly enabled the rapid multi-omic measurements needed to gain a better understanding of the members of each microbial community, while evaluating the changes taking place upon biological and environmental perturbations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.