ArticleJournal of visualized experiments : JoVE2018
Oral Bacterial Infection and Shedding in Drosophila melanogaster.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Drosophila melanogaster mitigates gastro-oral infections by stimulating pathogen expulsion.BMC microbiology · 2026Article
- Predicting bacterial-mediated entomopathogenicity through comparative genomics and statistical modeling.Microbiology spectrum · 2026Article
- Standard ingredient ofMicrobiology spectrum · 2025Article
- Investigating the immunomodulatory effects of honeybee venom peptide apamin inInfection and immunity · 2025Article
- Evolutionary History With Chronic Malnutrition Enhances Pathogen Susceptibility at Older Ages.Ecology and evolution · 2025Article
- Antimicrobial Effect of Diphenyl Ditelluride (PhTe)Indian journal of microbiology · 2024Article
- Genetic Variation in Trophic Avoidance Behaviour Shows Fruit Flies are Generally Attracted to Bacterial Substrates.Ecology and evolution · 2024Article
- IMD-mediated innate immune priming increases Drosophila survival and reduces pathogen transmission.PLoS pathogens · 2024Article
- Protein Restriction Effects on Healthspan and Lifespan in Drosophila melanogaster Are Additive With a Longevity-Promoting Diet.The journals of gerontology. Series A, Biological sciences and medical sciences · 2023Article
- Kinetics of blood cell differentiation during hematopoiesis revealed by quantitative long-term live imaging.eLife · 2023Article
- Ageing leads to reduced specificity of antimicrobial peptide responses inProceedings. Biological sciences · 2022Article
- Mechanisms of damage prevention, signalling and repair impact disease tolerance.Proceedings. Biological sciences · 2022Article
- Phenotyping ofBiomolecules · 2022Review
- Innate Immune Responses ofFrontiers in cellular and infection microbiology · 2021Article
- The Antiviral Effects of the Symbiont BacteriaFrontiers in immunology · 2020Review
- Sexual selection favours good or bad genes for pathogen resistance depending on males' pathogen exposure.Proceedings. Biological sciences · 2019Article
- Carry on caring: infected females maintain their parental care despite high mortality.Behavioral ecology : official journal of the International Society for Behavioral EcologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The fruit fly Drosophila melanogaster is one of the best developed model systems of infection and innate immunity. While most work has focused on systemic infections, there has been a recent increase of interest in the mechanisms of gut immunocompetence to pathogens, which require methods to orally infect flies. Here we present a protocol to orally expose individual flies to an opportunistic bacterial pathogen (Pseudomonas aeruginosa) and a natural bacterial pathogen of D. melanogaster (Pseudomonas entomophila). The goal of this protocol is to provide a robust method to expose male and female flies to these pathogens. We provide representative results showing survival phenotypes, microbe loads, and bacterial shedding, which is relevant for the study of heterogeneity in pathogen transmission. Finally, we confirm that Dcy mutants (lacking the protective peritrophic matrix in the gut epithelium) and Relish mutants (lacking a functional immune deficiency (IMD) pathway), show increased susceptibility to bacterial oral infection. This protocol, therefore, describes a robust method to infect flies using the oral route of infection, which can be extended to the study of a variety genetic and environmental sources of variation in gut infection outcomes and bacterial transmission.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.