Evidence map›Paper›PMID 29910875›Full record

ArticleChemical science2016

Activity modulation and allosteric control of a scaffolded DNAzyme using a dynamic DNA nanostructure.

Xiuhai Mao, Anna J Simon, Hao Pei, Jiye Shi, Jiang Li, Qing Huang, Kevin W Plaxco, Chunhai Fan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chemical science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Reactive oxygen species-resistant ultrastable super-resolution DNA framework dots.Proceedings of the National Academy of Sciences of the United States of America · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiuhai MaoDivision of Physical Biology & Bioimaging Center , Shanghai Synchrotron Radiation Facility , CAS Key Laboratory of Interfacial Physics and Technology , Shanghai Institute of Applied Physics , Chinese Academy of Sciences , Shanghai , China . Email: fchh@sinap.ac.cn.
Anna J SimonDepartment of Chemistry and Biomolecular Science and Engineering Program , University of California , Santa Barbara , California 93106 , USA.
Hao PeiDivision of Physical Biology & Bioimaging Center , Shanghai Synchrotron Radiation Facility , CAS Key Laboratory of Interfacial Physics and Technology , Shanghai Institute of Applied Physics , Chinese Academy of Sciences , Shanghai , China . Email: fchh@sinap.ac.cn.
Jiye ShiKellogg College , University of Oxford , Oxford , OX2 6PN , UK.
Jiang LiDivision of Physical Biology & Bioimaging Center , Shanghai Synchrotron Radiation Facility , CAS Key Laboratory of Interfacial Physics and Technology , Shanghai Institute of Applied Physics , Chinese Academy of Sciences , Shanghai , China . Email: fchh@sinap.ac.cn.
Qing HuangDivision of Physical Biology & Bioimaging Center , Shanghai Synchrotron Radiation Facility , CAS Key Laboratory of Interfacial Physics and Technology , Shanghai Institute of Applied Physics , Chinese Academy of Sciences , Shanghai , China . Email: fchh@sinap.ac.cn.
Kevin W PlaxcoDepartment of Chemistry and Biomolecular Science and Engineering Program , University of California , Santa Barbara , California 93106 , USA.
Chunhai FanDivision of Physical Biology & Bioimaging Center , Shanghai Synchrotron Radiation Facility , CAS Key Laboratory of Interfacial Physics and Technology , Shanghai Institute of Applied Physics , Chinese Academy of Sciences , Shanghai , China . Email: fchh@sinap.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recognition of the fundamental importance of allosteric regulation in biology dates back to not long after its discovery in the 1960s. Our ability to rationally engineer this potentially useful property into normally non-allosteric catalysts, however, remains limited. In response we report a DNA nanotechnology-enabled approach for introducing allostery into catalytic nucleic acids. Specifically, we have grafted one or two copies of a peroxidase-like DNAzyme, hemin-bound G-quadruplex (hemin-G), onto a DNA tetrahedral nanostructure in such a manner as to cause them to interact, modulating their catalytic activity. We achieve allosteric regulation of these catalysts by incorporating dynamically responsive oligonucleotides that respond to specific "effector" molecules (complementary oligonucleotides or small molecules), altering the spacing between the catalytic sites and thus regulating their activity. This designable approach thus enables subtle allosteric modulation in DNAzymes that is potentially of use for nanomedicine and nanomachines.

Identifiers

PMID29910875
PMCPMC5975834

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.