Evidence map›Paper›PMID 29910221›Full record

ArticleMicrobes and environments2018

Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments.

Yuki Morono, Tatsuhiko Hoshino, Takeshi Terada, Taketo Suzuki, Takahiro Sato, Hisashi Yuasa, Yuji Kubota, Fumio Inagaki

Abstract read
In one paragraph

Article in Microbes and environments, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yuki MoronoGeomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC).
Tatsuhiko HoshinoGeomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC).
Takeshi TeradaMarine Works Japan Ltd.
Taketo SuzukiKoken Ltd.
Takahiro SatoKoken Ltd.
Hisashi YuasaKoken Ltd.
Yuji KubotaKoken Ltd.
Fumio InagakiGeomicrobiology Group, Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology (JAMSTEC).

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.

Indexed as

AerosolsDNADNA ContaminationFiltrationHumansMolecular BiologyParticulate MatterPolymerase Chain ReactionQuality ControlRisk AssessmentAerosolsDNAParticulate MatterDNA contaminationexperimental quality controlultra-sensitive molecular approach

Identifiers

PMID29910221
PMCPMC6031387

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.