ArticleMethods in enzymology2018
Recent Advances in the Genetic Manipulation of Methylosinus trichosporium OB3b.
Article in Methods in enzymology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 22 citations in OpenAlex.
- Evading native restriction-modification systems improves electroporation efficiency of the methanotrophApplied and environmental microbiology · 2026Article
- Inverse stable isotope probing-metabolomics (InverSIP) identifies an iron acquisition system in a methane-oxidizing bacterial community.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biosynthesis of lanosterol inSynthetic and systems biotechnology · 2025Article
- Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox.Synthetic and systems biotechnology · 2024Article
- Identification of a TonB-Dependent Receptor Involved in Lanthanide Switch by the Characterization of Laboratory-Adapted Methylosinus trichosporium OB3b.Applied and environmental microbiology · 2023Article
- Systems analysis of the effect of hydrogen sulfide on the growth of Methylococcus capsulatus Bath.Applied microbiology and biotechnology · 2022Article
- Genome-Scale Metabolic Model Reconstruction and in Silico Investigations of Methane Metabolism inMicroorganisms · 2020Article
- Characterization of a long overlooked copper protein from methane- and ammonia-oxidizing bacteria.Nature communications · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Methanotrophic bacteria utilize methane as their sole carbon and energy source. Studies of the model Type II methanotroph Methylosinus trichosporium OB3b have provided insight into multiple aspects of methanotrophy, including methane assimilation, copper accumulation, and metal-dependent gene expression. Development of genetic tools for chromosomal editing was crucial for advancing these studies. Recent interest in methanotroph metabolic engineering has led to new protocols for genetic manipulation of methanotrophs that are effective and simple to use. We have incorporated these newer molecular tools into existing protocols for Ms. trichosporium OB3b. The modifications include additional shuttle and replicative plasmids as well as improved gene delivery and genotyping. The methods described here render gene editing in Ms. trichosporium OB3b efficient and accessible.
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Registered trials
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