Evidence map›Paper›PMID 29909608›Full record

ArticleCancer research and treatment2019

NFATC3-PLA2G15 Fusion Transcript Identified by RNA Sequencing Promotes Tumor Invasion and Proliferation in Colorectal Cancer Cell Lines.

Jee-Eun Jang, Hwang-Phill Kim, Sae-Won Han, Hoon Jang, Si-Hyun Lee, Sang-Hyun Song, Duhee Bang, Tae-You Kim

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer research and treatment, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

  1. Whole transcriptome analysis identifiesCancer pathogenesis and therapy · 2025
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  11. The Landscape of Actionable Gene Fusions in Colorectal Cancer.International journal of molecular sciences · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Jee-Eun JangCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Hwang-Phill KimCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Sae-Won HanCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Hoon JangDepartment of Chemistry, College of Science, Yonsei University, Seoul, Korea.
Si-Hyun LeeCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Sang-Hyun SongCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Duhee BangDepartment of Chemistry, College of Science, Yonsei University, Seoul, Korea.
Tae-You KimCancer Research Institute, Seoul National University College of Medicine, Seoul, Korea.
Seoul National University · KRSeoul National University Hospital · KRYonsei University · KR

Funding

Korea Health Industry Development InstituteMinistry of Health and Welfare 2013R 1A 2058552Ministry of Health and Welfare HI14C1277
6 · The paper itself

Abstract

purposeThis study was designed to identify novel fusion transcripts (FTs) and their functional significance in colorectal cancer (CRC) lines. MATERIALS AND

methodsWe performed paired-end RNA sequencing of 28 CRC cell lines. FT candidates were identified using TopHat-fusion, ChimeraScan, and FusionMap tools and further experimental validation was conducted through reverse transcription-polymerase chain reaction and Sanger sequencing. FT was depleted in human CRC line and the effects on cell proliferation, cell migration, and cell invasion were analyzed.

resultsOne thousand three hundred eighty FT candidates were detected through bioinformatics filtering. We selected six candidate FTs, including four inter-chromosomal and two intrachromosomal FTs and each FT was found in at least one of the 28 cell lines. Moreover, when we tested 19 pairs of CRC tumor and adjacent normal tissue samples, NFATC3-PLA2G15 FT was found in two. Knockdown of NFATC3-PLA2G15 using siRNA reduced mRNA expression of epithelial-mesenchymal transition (EMT) markers such as vimentin, twist, and fibronectin and increased mesenchymal-epithelial transition markers of E-cadherin, claudin-1, and FOXC2 in colo-320 cell line harboring NFATC3-PLA2G15 FT. The NFATC3-PLA2G15 knockdown also inhibited invasion, colony formation capacity, and cell proliferation.

conclusionThese results suggest that that NFATC3-PLA2G15 FTs may contribute to tumor progression by enhancing invasion by EMT and proliferation.

Indexed as

AcyltransferasesCell Line, TumorCell MovementCell ProliferationColorectal NeoplasmsEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHCT116 CellsHT29 CellsHumansNeoplasm InvasivenessNFATC Transcription FactorsOncogene Proteins, FusionPhospholipases A2Sequence Analysis, RNAAcyltransferasesNFATC3 protein, humanNFATC Transcription FactorsOncogene Proteins, Fusionphospholipase A2, group XVPhospholipases A2Colorectal neoplasmsFusion transcriptNFATC3PLA2G15RNA-sequencing

Identifiers

PMID29909608
PMCPMC6333966
OpenAlexW2808163099

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.