Trial reportScientific reports2018
Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease.
Trial report in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 4 syntheses or guidelines pooled it, 75 citations in OpenAlex.
- High-resolution melting analysis in veterinary medicine: a systematic review of applications and diagnostic accuracy.Tropical animal health and production · 2025Pooled it
- Diagnosis of human leptospirosis: systematic review and meta-analysis of the diagnostic accuracy of the Leptospira microscopic agglutination test, PCR targeting Lfb1, and IgM ELISA to Leptospira fainei serovar Hurstbridge.BMC infectious diseases · 2024Pooled it
- Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis.PLoS neglected tropical diseases · 2020Pooled it
- Nucleic acid and antigen detection tests for leptospirosis.The Cochrane database of systematic reviews · 2019Pooled it
- Genetic Diversity ofPathogens (Basel, Switzerland) · 2026Article
- Diagnostic Performance of Two Commercial qPCR Kits forTropical medicine and infectious disease · 2026Article
- Molecular identification and phylogenetic characterization ofJournal of advanced veterinary and animal research · 2026Article
- Urine PCR testing as an effective method for early diagnosis of leptospirosis.Microbiology spectrum · 2025Article
- One-pot, uracil-DNA-glycosylase-aided, and multi-indicator nanobiosensor detection platform for identifying Brucella melitensis from the Brucella spp.BMC microbiology · 2025Article
- Diagnostic Advances in Leptospirosis: A Comparative Analysis of Paraclinical Tests with a Focus on PCR.Microorganisms · 2025Review
- Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?Microorganisms · 2025Review
- Beyond serology: saccharide profiling enables identification of antigenically similarFrontiers in molecular biosciences · 2025Article
- Advancing serologic diagnosis: assessing the efficacy of rErpY-like protein in human leptospirosis detection.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Molecular surveillance of zoonotic pathogens from wild rodents in the Republic of Korea.PLoS neglected tropical diseases · 2024Article
- Pathological and molecular study of kidneys in apparently healthy cattle and sheep with special reference toVeterinary world · 2023Article
- Aptamer-based assay for rapid detection, surveillance, and screening of pathogenic Leptospira in water samples.Scientific reports · 2023Article
- One health approach on serosurvey of anti-One health (Amsterdam, Netherlands) · 2022Article
- Insight into the Structure, Functions, and Dynamics of theMembranes · 2022Review
- Effective microbial molecular diagnosis of periodontitis-related pathogen Porphyromonas gingivalis from salivary samples using rgpA gene.Genomics & informatics · 2022Article
- Enzyme immunoassays (EIA) for serodiagnosis of human leptospirosis: specific IgG3/IgG1 isotyping may further inform diagnosis of acute disease.PLoS neglected tropical diseases · 2022Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Currently, direct detection of Leptospira can be done in clinical laboratories by conventional and by real-time PCR (qRT-PCR). We tested a biobank of paired samples of serum and urine from the same patient (202 patients) presenting at the hospital in an area endemic for leptospirosis using qRT-PCR followed by high resolution melting (HRM) analysis. The results were compared with those obtained by conventional nested PCR and with the serologic gold standard microscopic agglutination test (MAT). Differences were resolved by sequencing. qRT-PCR-HRM was positive for 46 of the 202 patients (22.7%, accuracy 100%) which is consistent with known prevalence of leptospirosis in the Azores. MAT results were positive for 3 of the 46 patients (6.5%). Analysis of paired samples allowed us to identify the illness point at which patients presented at the hospital: onset, dissemination or excretion. The melting curve analysis of Leptospira species revealed that 60.9% (28/46) of patients were infected with L. interrogans and 39.1% (18/46) were infected with L. borgpetersenii, both endemic to the Azores. We validated the use of qRT-PCR-HRM for diagnosis of leptospirosis and for identification of the Leptospira species at the earliest onset of infection in a clinical setting, in less than 2 hours.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.