ArticleScientific reports2018
Ex vivo biomechanical characterization of syringe-needle ejections for intracerebral cell delivery.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Translational Considerations for Injectable Biomaterials and Bioscaffolds to Repair and Regenerate Brain Tissue.Advanced healthcare materials · 2026Review
- SIGMA: Shear-induced gelation by microbead aggregation in tubular flow systems.Materials today. Bio · 2026Article
- OPTRACE: Optical Imaging-Guided Transplantation and Tracking of Cells in the Mouse Brain.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Human induced pluripotent stem cell-derived therapies for regeneration after central nervous system injury.Neural regeneration research · 2025Article
- Development of self-healing hydrogels to support choroidal endothelial cell transplantation for the treatment of early age related macular degeneration.Acta biomaterialia · 2025Article
- The 3D-McMap Guidelines: Three-Dimensional Multicomposite Microsphere Adaptive Printing.Biomimetics (Basel, Switzerland) · 2024Article
- Evaluating the Feasibility of Hydrogel-Based Neural Cell Sprays.Journal of functional biomaterials · 2023Article
- The Potential of MSC-Based Cell-Free Therapy in Wound Healing-A Thorough Literature Review.International journal of molecular sciences · 2023Review
- 3D-Printed Microinjection Needle Arrays via a Hybrid DLP-Direct Laser Writing Strategy.Advanced materials technologies · 2023Article
- Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections.Journal of cell science & therapy · 2023Article
- Function and mechanism of mesenchymal stem cells in the healing of diabetic foot wounds.Frontiers in endocrinology · 2023Review
- Potential therapeutic strategies for photoreceptor degeneration: the path to restore vision.Journal of translational medicine · 2022Review
- Optimal Intravenous Administration Procedure for Efficient Delivery of Canine Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2022Article
- Cytoprotection of Human Progenitor and Stem Cells through Encapsulation in Alginate Templated, Dual Crosslinked Silk and Silk-Gelatin Composite Hydrogel Microbeads.Advanced healthcare materials · 2022Article
- Stem Cell-Based Therapy: A Promising Treatment for Diabetic Foot Ulcer.Biomedicines · 2022Review
- Translating cell therapies for neurodegenerative diseases: Huntington's disease as a model disorder.Brain : a journal of neurology · 2022Article
- Biomaterials to enhance stem cell transplantation.Cell stem cell · 2022Review
- Engineering Tissues of the Central Nervous System: Interfacing Conductive Biomaterials with Neural Stem/Progenitor Cells.Advanced healthcare materials · 2022Review
- Method of long-term, recurrent, intracerebroventricular infusion of cellular therapy in mice.Journal of neuroscience methods · 2022Article
- A Brief Review of In Vitro Models for Injury and Regeneration in the Peripheral Nervous System.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Intracerebral implantation of cell suspensions is finding its clinical translation with encouraging results in patients with stroke. However, the survival of cells in the brain remains poor. Although the biological potential of neural stem cells (NSCs) is widely documented, the biomechanical effects of delivering cells through a syringe-needle remain poorly understood. We here detailed the biomechanical forces (pressure, shear stress) that cells are exposed to during ejection through different sized needles (20G, 26G, 32G) and syringes (10, 50, 250 µL) at relevant flow rates (1, 5, 10 µL/min). A comparison of 3 vehicles, Phosphate Buffered Saline (PBS), Hypothermosol (HTS), and Pluronic, indicated that less viscous vehicles are favorable for suspension with a high cell volume fraction to minimize sedimentation. Higher suspension viscosity was associated with greater shear stress. Higher flow rates with viscous vehicle, such as HTS reduced viability by ~10% and also produced more apoptotic cells (28%). At 5 µL/min ejection using a 26G needle increased neuronal differentiation for PBS and HTS suspensions. These results reveal the biological impact of biomechanical forces in the cell delivery process. Appropriate engineering strategies can be considered to mitigate these effects to ensure the efficacious translation of this promising therapy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.