ArticleScientific reports2018
Inhibitory effect of SLIT2 on granulosa cell proliferation mediated by the CDC42-PAKs-ERK1/2 MAPK pathway in the prehierarchical follicles of the chicken ovary.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 50 citations in OpenAlex.
- CD47 stabilizes ROBO2 to regulate glioblastoma progression by preventing ITCH-mediated ubiquitination.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Micro RNA miR-726-3p targets CYB5A in Hen ovaries to modulate granulosa cell proliferation and differentiation.Scientific reports · 2025Article
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- Single-cell RNA sequencing uncovers dynamic roadmap during chicken spermatogenesis.BMC genomics · 2025Article
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- Whole-transcriptome insights into follicle selection: deciphering key regulatory networks in Luxi gamecock.Frontiers in genetics · 2025Article
- Slit1 inhibits ovarian follicle development and female fertility in mice†.Biology of reproduction · 2024Article
- Long non-coding RNAFrontiers in veterinary science · 2024Article
- The genomic characteristics affect phenotypic diversity from the perspective of genetic improvement of economic traits.iScience · 2023Article
- Transcriptomic analysis of ovarian follicles uncovers the crucial genes relevant to follicle selection and preovulatory hierarchy in hens.Journal of animal science · 2023Article
- Knockdown of CLAUDIN-6 Inhibited Apoptosis and Induced Proliferation of Bovine Cumulus Cells.International journal of molecular sciences · 2022Article
- The study of candidate genes in the improvement of egg production in ducks - a review.Poultry science · 2022Review
- Advances in the study of CDC42 in the female reproductive system.Journal of cellular and molecular medicine · 2022Review
- Transcriptome comparative analysis of ovarian follicles reveals the key genes and signaling pathways implicated in hen egg production.BMC genomics · 2021Article
- Genetic Basis of Follicle Development in Dazu Black Goat by Whole-Transcriptome Sequencing.Animals : an open access journal from MDPI · 2021Article
- Slit/Robo signaling regulates Leydig cell steroidogenesis.Cell communication and signaling : CCS · 2021Article
- WGCNA Analysis Identifies Polycystic Ovary Syndrome-Associated Circular RNAs That Interact with RNA-Binding Proteins and Sponge miRNAs.International journal of general medicine · 2021Article
- System-Level Analysis of Alzheimer's Disease Prioritizes Candidate Genes for Neurodegeneration.Frontiers in genetics · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SLIT2 ligand and ROBO receptors of the SLIT/ROBO pathway are expressed in hen ovarian follicles and have been shown to play critical roles in ovary development, cell proliferation and apoptosis in mammals. However, the exact roles of SLIT2 and the molecular mechanisms of chicken follicle development remain poorly understood. Here, we discovered that high levels of SLIT2 suppress FSHR, GDF9, STAR and CYP11A1 mRNA and protein expression in granulosa cells (GCs) and cell proliferation (p < 0.01). However, these inhibitory effects can be abolished by the siRNA-mediated knockdown of the ROBO1 and ROBO2 receptors. Furthermore, the activity of CDC42, which is a key Rho GTPase in the SLIT/ROBO pathway, is regulated by the ligand SLIT2 because the intrinsic GTPase activation activity of CDC42 is activated or repressed by regulating SRGAP1 expression (p < 0.01). The effects of the SLIT2 overexpression on GC proliferation and phosphorylation of the B-RAF, RAF1 and ERK1/2 kinases were completely abrogated by knocking down endogenous PAK1 and partially abrogated by the knockdown of PAK2 and PAK3 in the GCs. Collectively, our findings indicate that SLIT2 suppresses GC proliferation, differentiation and follicle selection mainly by a mechanism involving ROBO1 and ROBO2 and that this suppression is mediated by the CDC42-PAKs-ERK1/2 MAPK signaling cascade in the prehierarchical follicles of the chicken ovary.
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