ArticleScientific reports2018
Accurate and precise real-time RT-PCR assays for the identification of astrovirus associated encephalitis in cattle.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Phylogenomic analysis of bovine astrovirus expands the known repertoire of mamastrovirus genotypes in northern India.Archives of virology · 2025Article
- Bovine astrovirus and its role in lymphocytic encephalitis in cattle in Ontario, Canada, 1988-2019.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Development of a one-step multiplex RT-qPCR method for rapid detection of bovine diarrhea viruses.Frontiers in cellular and infection microbiology · 2024Article
- Bovine Astrovirus-A Comprehensive Review.Viruses · 2022Review
- Article
- Detection of Astrovirus in a Cow with Neurological Signs by Nanopore Technology, Italy.Viruses · 2020Article
- Fecal Shedding of Bovine Astrovirus CH13/NeuroS1 in Veal Calves.Journal of clinical microbiology · 2020Article
- Parainfluenza Virus 5 Infection in Neurological Disease and Encephalitis of Cattle.International journal of molecular sciences · 2020Article
- Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives.Acta veterinaria Scandinavica · 2019Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel bovine astrovirus genotype species (BoAstV-CH13/NeuroS1) was recently identified in brain tissues of cattle as a plausible cause of encephalitis. The purpose of the present study was to develop and validate real time RT-PCR assays for the detection of BoAstV-CH13/NeuroS1 in brain tissues of cattle. Three different primer-probe combinations were designed based on BoAstV-CH13/NeuroS1 full-genome sequences of 11 different strains identified in cattle, and established in three distinct one-step real time RT-PCR protocols. These protocols were compared regarding their diagnostic performance using brain tissues of cattle with and without astrovirus associated encephalitis. The limit of detection (LOD) of all three assays was between 1.34 × 10
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