ArticleMicrobial cell factories2018
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with L-isoleucine in Corynebacterium glutamicum WM001.
Article in Microbial cell factories, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 49 citations in OpenAlex.
- Potential sustained production of polyhydroxyalkanoate copolymers using cyanobacteria microbiomes.Scientific reports · 2025Article
- Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.Molecules (Basel, Switzerland) · 2024Review
- Comprehensive analysis of genomic variation, pan-genome and biosynthetic potential of Corynebacterium glutamicum strains.PloS one · 2024Article
- Copolymers and Blends Based on 3-Hydroxybutyrate and 3-Hydroxyvalerate Units.International journal of molecular sciences · 2023Review
- Rapid combinatorial rewiring of metabolic networks for enhanced poly(3-hydroxybutyrate) production in Corynebacterium glutamicum.Microbial cell factories · 2023Article
- Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids.Microbial cell factories · 2021Review
- Engineering the Outer Membrane Could Facilitate Better Bacterial Performance and Effectively Enhance Poly-3-Hydroxybutyrate Accumulation.Applied and environmental microbiology · 2021Article
- Construction of an Escherichia coli Strain Lacking Fimbriae by Deleting 64 Genes and Its Application for Efficient Production of Poly(3-Hydroxybutyrate) and l-Threonine.Applied and environmental microbiology · 2021Article
- Effect of sodium dodecyl sulfate on the production of L-isoleucine by the fermentation ofBioengineered · 2020Article
- Biodegradable Polymeric Materials: Synthetic Approach.ACS omega · 2020Review
- Comprehensive Analysis ofFrontiers in bioengineering and biotechnology · 2020Article
- Increasing L-threonine production in Escherichia coli by overexpressing the gene cluster phaCAB.Journal of industrial microbiology & biotechnology · 2019Article
- Construction of Efficient PlatformPolymers · 2019Article
- Bromination of L-tryptophan in a Fermentative Process WithFrontiers in bioengineering and biotechnology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCo-production of polyhydroxyalkanoate (PHA) and amino acids makes bacteria effective microbial cell factories by secreting amino acids outside while accumulating PHA granules inside. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is one of the PHAs with biocompatibility and fine mechanical properties, but its production is limited by the low level of intracellular propionyl-CoA.
resultsL-Isoleucine producing Corynebacterium glutamicum strain WM001 were analyzed by genome and transcriptome sequencing. The results showed that the most over-expressed genes in WM001 are relevant not only to L-isoleucine production but also to propionyl-CoA accumulation. Compared to the wild-type C. glutamicum ATCC13869, the transcriptional levels of the genes prpC2, prpD2, and prpB2, which are key genes relevant to propionyl-CoA accumulation, increased 2
conclusionsGenome and transcriptome analysis showed that C. glutamicum WM001 has potential to accumulate L-isoleucine and propionyl-CoA pool. This was experimentally confirmed by introducing the phaCAB gene cluster into WM001. The recombinant strain WM001/pDXW-8-phaCAB produced high levels of PHBV with high 3-hydroxyvalerate fraction as well as L-isoleucine. Because of its high level of intracellular propionyl-CoA pool, WM001 might be used for producing other propionyl-CoA derivatives.
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