Evidence map›Paper›PMID 29906704›Full record

ArticleVirology2018

Conserved cysteines in Mason-Pfizer monkey virus capsid protein are essential for infectious mature particle formation.

Růžena Píchalová, Tibor Füzik, Barbora Vokatá, Michaela Rumlová, Manuel Llano, Alžběta Dostálková, Ivana Křížová, Tomáš Ruml, Pavel Ulbrich

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Růžena PíchalováDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: pichalor@vscht.cz.
Tibor FüzikDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: fuzikt@vscht.cz.
Barbora VokatáDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: vokataa@vscht.cz.
Michaela RumlováDepartment of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: rumlovai@vscht.cz.
Manuel LlanoDepartment of Biological Sciences, University of Texas at El Paso, 500 West University El Paso, TX 79902, USA. Electronic address: mllano@utep.edu.
Alžběta DostálkováDepartment of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: dostalkl@vscht.cz.
Ivana KřížováDepartment of Biotechnology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: krizovaa@vscht.cz.
Tomáš RumlDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: rumlt@vscht.cz.
Pavel UlbrichDepartment of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic. Electronic address: ulbrichp@vscht.cz.
University of Chemistry and Technology, Prague · CZThe University of Texas at El Paso · US

Funding

Role of PARP-1 in HIV-1 latent infectionSC1GM115240 · NIGMS · UNIVERSITY OF TEXAS EL PASO · PI LLANO, MANUEL · 2016 to 2019
$1.5M
NIGMS NIH HHS SC1 GM115240
6 · The paper itself

Abstract

Retrovirus assembly is driven mostly by Gag polyprotein oligomerization, which is mediated by inter and intra protein-protein interactions among its capsid (CA) domains. Mason-Pfizer monkey virus (M-PMV) CA contains three cysteines (C82, C193 and C213), where the latter two are highly conserved among most retroviruses. To determine the importance of these cysteines, we introduced mutations of these residues in both bacterial and proviral vectors and studied their impact on the M-PMV life cycle. These studies revealed that the presence of both conserved cysteines of M-PMV CA is necessary for both proper assembly and virus infectivity. Our findings suggest a crucial role of these cysteines in the formation of infectious mature particles.

Indexed as

Virus AssemblyCapsid ProteinsCell LineCysteineGenetic VectorsHEK293 CellsHumansMason-Pfizer monkey virusMutationProvirusesVirionCapsid ProteinsCysteineCysteine mutagenesisM-PMV capsidM-PMV infectivityRetrovirus assemblyVirus core stability

Identifiers

PMID29906704
PMCPMC6379149
OpenAlexW2807841448

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.