ArticleBiochemistry2018
X-ray Emission Spectroscopy as an in Situ Diagnostic Tool for X-ray Crystallography of Metalloproteins Using an X-ray Free-Electron Laser.
Article in Biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Article
- An additional water is introduced into the manganese cluster during the formation of the SNature communications · 2026Article
- Coupled on-line in crystallo UV-Vis absorption spectroscopy and X-ray crystallography to compare specific radiation damage in metal-containing proteins at room versus cryogenic temperature.Acta crystallographica. Section D, Structural biology · 2026Article
- Structural basis of the promiscuity of the unusual Fe(II) and 2-oxoglutarate dependent human aspartate/asparagine-β-hydroxylase.Nature communications · 2026Article
- Can ferric-oxyl excited states explain elongated iron-oxygen bonds in heme peroxidase catalytic intermediates?Nature communications · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.Nature communications · 2024Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Evolutionary diversity of proton and water channels on the oxidizing side of photosystem II and their relevance to function.Photosynthesis research · 2023Review
- Going around the Kok cycle of the water oxidation reaction with femtosecond X-ray crystallography.IUCrJ · 2023Article
- Combining on-line spectroscopy with synchrotron and X-ray free electron laser crystallography.Current opinion in structural biology · 2023Review
- Does Serial Femtosecond Crystallography Depict State-Specific Catalytic Intermediates of the Oxygen-Evolving Complex?Journal of the American Chemical Society · 2023Article
- Structural evidence for intermediates during ONature · 2023Article
- X-ray Emission Spectroscopy of Single Protein Crystals Yields Insights into Heme Enzyme Intermediates.The journal of physical chemistry letters · 2023Article
- Capturing the sequence of events during the water oxidation reaction in photosynthesis using XFELs.FEBS letters · 2023Review
- Room temperature crystallography and X-ray spectroscopy of metalloenzymes.Methods in enzymology · 2023Article
- Determination of the iron(IV) local spin states of the Q intermediate of soluble methane monooxygenase by Kβ X-ray emission spectroscopy.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2022Article
- AXEAP: a software package for X-ray emission data analysis using unsupervised machine learning.Journal of synchrotron radiation · 2022Article
- Serial femtosecond crystallography.Nature reviews. Methods primers · 2022Article
- XFEL serial crystallography reveals the room temperature structure of methyl-coenzyme M reductase.Journal of inorganic biochemistry · 2022Article
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12 authors.
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Abstract
Serial femtosecond crystallography (SFX) using the ultrashort X-ray pulses from a X-ray free-electron laser (XFEL) provides a new way of collecting structural data at room temperature that allows for following the reaction in real time after initiation. XFEL experiments are conducted in a shot-by-shot mode as the sample is destroyed and replenished after each X-ray pulse, and therefore, monitoring and controlling the data quality by using in situ diagnostic tools is critical. To study metalloenzymes, we developed the use of simultaneous collection of X-ray diffraction of crystals along with X-ray emission spectroscopy (XES) data that is used as a diagnostic tool for crystallography, by monitoring the chemical state of the metal catalytic center. We have optimized data analysis methods and sample delivery techniques for fast and active feedback to ensure the quality of each batch of samples and the turnover of the catalytic reaction caused by reaction triggering methods. Here, we describe this active in situ feedback system using Photosystem II as an example that catalyzes the oxidation of H
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