ArticleAnnals of botany2018
Isolation and functional characterization of CsLsi2, a cucumber silicon efflux transporter gene.
Article in Annals of botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Is Silicon a Panacea for Alleviating Drought and Salt Stress in Crops?Frontiers in plant science · 2020Pooled it
- Emerging roles of silicon in plant signaling networks and microbiome dynamics under salt stress.Frontiers in plant science · 2026Review
- Characterization of two proline-rich proteins involved in silicon deposition inFrontiers in plant science · 2025Article
- The Physiological and Molecular Mechanisms of Silicon Action in Salt Stress Amelioration.Plants (Basel, Switzerland) · 2024Review
- A silicon transporter gene required for healthy growth of rice on land.Nature communications · 2023Article
- Lsi2: A black box in plant silicon transport.Plant and soil · 2021Review
- Role of Silicon in Mediating Phosphorus Imbalance in Plants.Plants (Basel, Switzerland) · 2020Review
- Silicon Enhances Biomass and Grain Yield in an Ancient Crop Tef [Frontiers in plant science · 2020Article
- Genome-wide identification, structure characterization, and expression pattern profiling of aquaporin gene family in cucumber.BMC plant biology · 2019Article
- Role of Silicon in Mediating Salt Tolerance in Plants: A Review.Plants (Basel, Switzerland) · 2019Review
- A Review on Si Uptake and Transport System.Plants (Basel, Switzerland) · 2019Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and Aims: Silicon has been proven to exert beneficial effects on plant growth and stress tolerance, and silicon accumulation varies among different plant species. Cucumber (Cucumis sativus) is a widely used dicot model for silicon accumulation, but little is known about the molecular mechanism of its silicon uptake. Previously, we isolated and characterized CsLsi1, a silicon influx transporter gene from cucumber. In this study, we cloned a putative silicon efflux transporter gene, CsLsi2, from cucumber and investigated its role in silicon uptake. Methods: The expression pattern, transport activity, and subcellular and cellular localizations of CsLsi2 were investigated. The transport activity of CsLsi2 was determined in Xenopus laevis oocytes. The subcelluar and cellular localizations were conducted by transient expression of fused 35S::CsLsi2-eGFP in onion epidermal cells and expression of ProCsLsi2::CsLsi2-mGFP in cucumber, respectively. Key Results: CsLsi2 was mainly expressed in the roots. Expression of CsLsi2-eGFP fusion sequence in onion epidermis cells showed that CsLsi2 was localized at the plasma membrane. Transient expression in Xenopus laevis oocytes showed that CsLsi2 demonstrated efflux but no influx transport activity for silicon, and the transport was energy-dependent. Expression of CsLsi2-mGFP under its own promoter revealed that CsLsi2 was mainly expressed on endodermal cells, showing no polar distribution. In combination with our previous work on CsLsi1, a model for silicon uptake in cucumber roots is proposed. Conclusion: The results suggest that CsLsi2 is a silicon efflux transporter gene in cucumber. The coordination of CsLsi1 and CsLsi2 mediates silicon uptake in cucumber roots. This study may help us understand the molecular mechanism for silicon uptake in cucumber, one of the few dicots with a relatively high capacity for silicon accumulation.
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