ArticleRNA (New York, N.Y.)2018
Influenza A virus-derived siRNAs increase in the absence of NS1 yet fail to inhibit virus replication.
Article in RNA (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Bat Dicer antiviral role and subcellular localization differ upon alphavirus infection in two distinct species.PLoS pathogens · 2025Article
- The helicase domain of human Dicer prevents RNAi-independent activation of antiviral and inflammatory pathways.The EMBO journal · 2024Article
- Delivery of siRNAs against MERS-CoV in Vero and HEK-293 cells: A comparative evaluation of transfection reagents.Journal of King Saud University. Science · 2023Article
- Computational Design and Experimental Evaluation of MERS-CoV siRNAs in Selected Cell Lines.Diagnostics (Basel, Switzerland) · 2023Article
- Detection of SARS-CoV-2 Derived Small RNAs and Changes in Circulating Small RNAs Associated with COVID-19.Viruses · 2021Article
- Efficient Dicer processing of virus-derived double-stranded RNAs and its modulation by RIG-I-like receptor LGP2.PLoS pathogens · 2021Article
- An isoform of Dicer protects mammalian stem cells against multiple RNA viruses.Science (New York, N.Y.) · 2021Article
- Human DICER helicase domain recruits PKR and modulates its antiviral activity.PLoS pathogens · 2021Article
- In Vitro Inhibitory Analysis of Rationally Designed siRNAs against MERS-CoV Replication in Huh7 Cells.Molecules (Basel, Switzerland) · 2021Article
- Article
- Functional Mapping of AGO-Associated Zika Virus-Derived Small Interfering RNAs in Neural Stem Cells.Frontiers in cellular and infection microbiology · 2021Article
- Article
- A Requirement for Argonaute 4 in Mammalian Antiviral Defense.Cell reports · 2020Article
- Comparative mRNA and LncRNA Analysis of the Molecular Mechanisms Associated With Low Silk Production inFrontiers in genetics · 2020Article
- Defective Viral Particles Produced in Mast Cells Can Effectively Fight Against Lethal Influenza A Virus.Frontiers in microbiology · 2020Article
- Review
- Article
- Slicing and dicing viruses: antiviral RNA interference in mammals.The EMBO journal · 2019Review
- Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids.Cell research · 2019Article
- Main constraints for RNAi induced by expressed long dsRNA in mouse cells.Life science alliance · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
While the issue of whether RNA interference (RNAi) ever forms part of the antiviral innate immune response in mammalian somatic cells remains controversial, there is considerable evidence demonstrating that few, if any, viral small interfering RNAs (siRNAs) are produced in infected cells. Moreover, inhibition of RNAi by mutational inactivation of key RNAi factors, such as Dicer or Argonaute 2, fails to enhance virus replication. One potential explanation for this lack of inhibitory effect is that mammalian viruses encode viral suppressors of RNAi (VSRs) that are so effective that viral siRNAs are not produced in infected cells. Indeed, a number of mammalian VSRs have been described, of which the most prominent is the influenza A virus (IAV) NS1 protein, which has not only been reported to inhibit RNAi in plants and insects but also to prevent the production of viral siRNAs in IAV-infected human cells. Here, we confirm that an IAV mutant lacking NS1 indeed differs from wild-type IAV in that it induces the production of readily detectable levels of Dicer-dependent viral siRNAs in infected human cells. However, we also demonstrate that these siRNAs have little if any inhibitory effect on IAV gene expression. This is likely due, at least in part, to their inefficient loading into RNA-induced silencing complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.