Evidence map›Paper›PMID 29901762›Full record

ArticleNucleic acids research2018

3'-End labeling of nucleic acids by a polymerase ribozyme.

Biswajit Samanta, David P Horning, Gerald F Joyce

Abstract read
In one paragraph

Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Tag-Free Internal RNA Labeling and Photocaging Based on mRNA Methyltransferases.Angewandte Chemie (International ed. in English) · 2021
    Article
  7. Covalent labeling of nucleic acids.Chemical Society reviews · 2020
    Review
  8. Article
  9. Site-Selective RNA Functionalization via DNA-Induced Structure.Journal of the American Chemical Society · 2020
    Article
  10. Repurposing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling of RNA.Angewandte Chemie (International ed. in English) · 2020
    Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Biswajit SamantaThe Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
David P HorningThe Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Gerald F JoyceThe Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A polymerase ribozyme can be used to label the 3' end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs. Guided by a complementary template, the ribozyme adds a single nucleotide that may contain a fluorophore, biotin, azide or alkyne moiety, thus enabling the detection and/or capture of selectively labeled materials. Employing a variety of commercially available nucleotide analogs, efficient labeling was demonstrated for model RNAs and DNAs, human microRNAs and natural tRNA.

Indexed as

3' Flanking RegionBiotinDNADNA-Directed RNA PolymerasesFluoresceinFluorescent DyesMicroRNAsNucleic Acid ConformationNucleic AcidsNucleotidyltransferasesRhodaminesRNARNA, CatalyticRNA, TransferStaining and LabelingBiotinDNADNA-Directed RNA PolymerasesFluoresceinFluorescent DyesMicroRNAsNucleic AcidsNucleotidyltransferasesRhodaminesRNARNA, CatalyticRNA, Transfer

Identifiers

PMID29901762
PMCPMC6158495

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.