Evidence map›Paper›PMID 29900094›Full record

ArticleEuPA open proteomics2016

Tue Bjerg Bennike, Kenneth Kastaniegaard, Simona Padurariu, Michael Gaihede, Svend Birkelund, Vibeke Andersen, Allan Stensballe

Abstract read
In one paragraph

Article in EuPA open proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Sample Collection Methods in Upper Gastrointestinal Research.Journal of Korean medical science · 2023
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Assessment of RNAlaterFoods (Basel, Switzerland) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tue Bjerg BennikeResearch Unit for Molecular Diagnostic and Clinical Research, Hospital of Southern Jutland, Aabenraa, Denmark.
Kenneth KastaniegaardDepartment of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Simona PadurariuDepartment of Otolaryngology, Head and Neck Surgery, Aalborg University Hospital, Aalborg, Denmark.
Michael GaihedeDepartment of Otolaryngology, Head and Neck Surgery, Aalborg University Hospital, Aalborg, Denmark.
Svend BirkelundDepartment of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Vibeke AndersenResearch Unit for Molecular Diagnostic and Clinical Research, Hospital of Southern Jutland, Aabenraa, Denmark.
Allan StensballeDepartment of Health Science and Technology, Aalborg University, Aalborg, Denmark.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Large biobanks exist worldwide containing formalin-fixed, paraffin-embedded samples and samples stored in RNAlater. However, the impact of tissue preservation on the result of a quantative proteome analysis remains poorly described. Human colon mucosal biopsies were extracted from the sigmoideum and either immediately frozen, stabilized in RNAlater, or stabilized by formalin-fixation. In one set of biopsies, formalin stabilization was delayed for 30 min. The protein content of the samples was characterized by high throughput quantitative proteomics. We were able to identify a similar high number of proteins in the samples regardless of preservation method, with only minor differences in protein quantitation.

Indexed as

CAN, acetonitrileDF, directly-frozenFA, formic acidFASP, filter-aided sample preparationFDR, false discovery rateFFPE, formalin-fixedFormalin-fixedHLA-A class I, histocompatibility antigen A-23 alpha chainHLA-DRB1 class II, histocompatibility antigen DRB1-4 beta chainHuman colon mucosaiFFPE, immediately formalin-fixedLFQ, label-free quantificationMass spectrometryParaffin-embeddedPCA, principle component analysisPreservationProteomicsPSM, peptide spectral matchPTM, post-translational modificationRNAlaterSDC, sodium deoxycholateSDS, sodium dodecyl sulfatesFFPE, stored for 30 min prior to formalin-fixeds, standard deviationTEAB, triethylammonium bicarbonate

Identifiers

PMID29900094
PMCPMC5988570

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.