Proteomic comparison of the EWS-FLI1 expressing cells EF with NIH-3T3 and actin remodeling effect of (R/W)

Séverine Clavier, Françoise Illien, Sandrine Sagan, Gérard Bolbach, Emmanuelle Sachon

Open access · goldAbstract read

In one paragraph

Article in EuPA open proteomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact, top 95% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Séverine ClavierSorbonne Université, UPMC-Univ Paris 6, Ecole Normale Supérieure, PSL Research University, Département de Chimie, CNRS, UMR7203 Laboratoire des BioMolécules, 4 Place Jussieu, Paris Cedex 05, 75252 Paris, France.

Françoise IllienSorbonne Université, UPMC⿿Univ Paris 6, Plateforme de Spectrométrie de Masse et Protéomique-IBPS, cc41, 7-9 Quai Saint Bernard, Paris Cedex 05, 75252 Paris, France.

Sandrine SaganSorbonne Université, UPMC⿿Univ Paris 6, Plateforme de Spectrométrie de Masse et Protéomique-IBPS, cc41, 7-9 Quai Saint Bernard, Paris Cedex 05, 75252 Paris, France.

Gérard BolbachSorbonne Université, UPMC-Univ Paris 6, Ecole Normale Supérieure, PSL Research University, Département de Chimie, CNRS, UMR7203 Laboratoire des BioMolécules, 4 Place Jussieu, Paris Cedex 05, 75252 Paris, France.

Emmanuelle SachonSorbonne Université, UPMC-Univ Paris 6, Ecole Normale Supérieure, PSL Research University, Département de Chimie, CNRS, UMR7203 Laboratoire des BioMolécules, 4 Place Jussieu, Paris Cedex 05, 75252 Paris, France.

Physique des interactions ioniques et moléculaires · FRSorbonne Université · FRÉcole Normale Supérieure - PSL · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

EWS-FLI1 expression in NIH-3T3 fibroblasts has a profound impact on the phenotype, resulting in the cytoskeleton and adhesive capacity disorganization (EF cells). Besides this, (R/W) BIOLOGICAL SIGNIFICANCE: Up to now very few global quantitative proteomic studies have been published to help understand the oncogenic transformation induced by EWS-FLI1 fusion protein and leading to Ewing sarcoma development and dissemination. The comparison we did in this study between a model tumoral cell line EF and its non-tumoral counterpart (3T3) allowed us to highlight several features either common to most tumor types or specific to Ewing sarcoma. Particularly, lack of actin cytoskeleton organization could very likely be explained by the down-regulation of many important actin binding proteins. These results are in accordance with the hypothesis of a passive/stochastic mode of dissemination conferring Ewing sarcoma tumoral cell a high metastatic potential.

Indexed as

Actin cytoskeleton remodelingCell-penetrating peptideEWS-FLI1Passive disseminationSILAC quantitative proteomic approach

Identifiers

PMID29900093

DOI10.1016/j.euprot.2015.10.002

PMCPMC5988571

OpenAlexW1909370489

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.