Evidence map›Paper›PMID 29899566›Full record

ArticleScientific reports2018

GATA-4-expressing mouse bone marrow mesenchymal stem cells improve cardiac function after myocardial infarction via secreted exosomes.

Ji-Gang He, Hong-Rong Li, Jin-Xiu Han, Bei-Bei Li, Dan Yan, Hong-Yuan Li, Ping Wang, Ying Luo

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 45 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed, 2 pooled it
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 2 syntheses or guidelines pooled it, 79 citations in OpenAlex.

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  10. The Potential of Mesenchymal Stem Cell-Derived Exosomes in Cardiac Repair.International journal of molecular sciences · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Ji-Gang HeDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China.
Hong-Rong LiDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China.
Jin-Xiu HanDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China.
Bei-Bei LiDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China.
Dan YanDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China.
Hong-Yuan LiDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China.
Ping WangDepartment of Cardiac and Vascular Surgery, First People's Hospital of Yunnan Province, No. 157 Jinbi Road, Kunming, Yunnan Province, 650032, China. jiganghe@sina.com.
Ying LuoKunming University of Science and Technology, NO.68, Wenchang Road, 121 Street, Kunming, Yunnan Province, 650032, China. jiganghe999@163.com.
First People's Hospital of Yunnan Province · CNKunming University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to investigate whether exosomes secreted by mouse GATA-4-expressing bone marrow mesenchymal stem cells (BMSCs) could induce BMSC differentiation into myocyte precursors, decrease cardiomyocyte apoptosis, and improve cardiac function following myocardial infarction (MI). BMSCs were transduced with a lentivirus carrying a doxycycline (DOX)-inducible GATA-4 or control lentivirus, and secreted exosomes from these BMSCs were collected and co-cultured with BMSCs or cardiomyocytes under hypoxic and serum free conditions. Furthermore, exosomes were injected into mice 48 h after MI. Cardiac function was evaluated by echocardiography at 48, 72, and 96 h after exosome treatment. Quantitative PCR showed that co-culture of BMSCs with GATA-4-BMSC exosomes increased cardiomyocyte-related marker expression. Co-culture of GATA-4-BMSC exosomes with cardiomyocytes in anoxic conditions decreased apoptosis as detected by flow cytometry. Injection of GATA-4-BMSC exosomes in mice 48 h after MI increased cardiac function over the next 96 h; increased cardiac blood vessel density and number of c-kit-positive cells and decreased apoptotic cardiomyocyte cells were also observed. Differential expression of candidate differentiation- and apoptosis-related miRNAs and proteins that may mediate these effects was also identified. Exosomes isolated from GATA-4-expressing BMSCs induce differentiation of BMSCs into cardiomyocyte-like cells, decrease anoxia-induced cardiomyocyte apoptosis, and improve myocardial function after infarction.

Indexed as

AnimalsApoptosisCell DifferentiationCell HypoxiaCells, CulturedCoculture TechniquesExosomesGATA4 Transcription FactorGene ExpressionHeartMaleMesenchymal Stem CellsMesenchymal Stem Cell TransplantationMice, Inbred C57BLMyocardial InfarctionMyocytes, CardiacGATA4 Transcription Factor

Identifiers

PMID29899566
PMCPMC5998064
OpenAlexW2807504811

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.