Evidence map›Paper›PMID 29898783›Full record

ArticleBMC research notes2018

Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase.

Marina Ioannou, Dimitris N Papageorgiou, Vasily Ogryzko, John Strouboulis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Marina IoannouInstitute of Molecular Biology and Biotechnology, Foundation of Research & Technology Hellas, 100 Nikolaou Plastira Street, 70013, Heraklion, Crete, Greece.
Dimitris N PapageorgiouDivision of Molecular Oncology, Biomedical Sciences Research Center "Alexander Fleming", 34 Fleming Street, 166 72, Vari, Greece.
Vasily OgryzkoUMR8126, Université Paris-Sud 11, CNRS, Institut de Cancérologie Gustave Roussy, 94805, Villejuif, France.
John StrouboulisInstitute of Molecular Biology and Biotechnology, Foundation of Research & Technology Hellas, 100 Nikolaou Plastira Street, 70013, Heraklion, Crete, Greece. john_strouboulis@imbb.forth.gr.
Foundation for Research and Technology Hellas · GRAlexander Fleming Biomedical Sciences Research Center · GRUniversité Paris-Sud · FR

Funding

Functional proteomics in differentiating erythroid cellsR01DK083389 · NIDDK · UNIVERSITY OF FLORIDA · PI BUNGERT, JORG, STROUBOULIS, IOANNIS JOHN · 2010 to 2013
$1.1M
National Institute of Diabetes and Digestive and Kidney Diseases RO1DK083389NIDDK NIH HHS R01 DK083389
6 · The paper itself

Abstract

ΟBJECTIVE: To construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag.

resultsWe constructed and tested by transient transfections mammalian expression vectors for the co-expression from a single plasmid of N- or C-terminally tagged proteins bearing a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag separated by a tobacco etch virus (TEV) protease cleavage site, together with a mammalian codon-optimized BirA biotin ligase fused to green fluorescent protein. We also describe platform vectors for the N- or C-terminal AVI-TEV-FLAG tagging of any complementary DNA of choice. These vectors offer versatility and efficiency in the application of metabolic biotinylation tandem affinity tagging of nuclear proteins in mammalian cells.

Indexed as

Affinity LabelsGenetic VectorsAnimalsBiotinylationHEK293 CellsHumansMicePlasmidsRabbitsRatsAffinity LabelsAvi tagBirA biotin ligaseExpression vectorsFLAG tagMetabolic biotinylation taggingTandem affinity purification

Identifiers

PMID29898783
PMCPMC6001059
OpenAlexW2807934605

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.