ArticleJournal of applied oral science : revista FOB2018
Standardization of a protocol for shotgun proteomic analysis of saliva.
Article in Journal of applied oral science : revista FOB, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- High Gingival Bleeding Burden in Late Pregnancy Is Associated with a Salivary Profile of Inflammatory, Regulatory, and Stress-Response Signals.Life (Basel, Switzerland) · 2026Article
- Dietary patterns characterized by high fiber intake in vegan and omnivorous individuals are associated with distinct salivary proteomic signatures linked to oral homeostasis.Clinical oral investigations · 2026Article
- Salivary Proteomic Signatures in Pregnant Women With Excessive Gingival Bleeding.Oral diseases · 2026Observational
- Salivary Proteome Role in Infection and Immunity.Advances in experimental medicine and biology · 2026Review
- Global Salivary Proteomic Profiling of Individuals with the Co-Occurrence of Type 2 Diabetes Mellitus, Dyslipidemia, and Periodontitis.International journal of molecular sciences · 2025Article
- Natural and induced immune responses in oral cavity and saliva.BMC immunology · 2025Review
- Salivary Proteome Insights: Evaluation of Saliva Preparation Methods in Mucopolysaccharidoses Research.Biomedicines · 2025Article
- CHANGES IN SALIVARY PROTEOME WITH REGARD TO PAIN-RELATED PROTEINS FOLLOWING FIXED ORTHODONTIC APPLIANCE PLACEMENT.Acta clinica Croatica · 2025Article
- Article
- Generalized gingivitis-related salivary proteomic profile in pregnant women with obesity: insights into biological mechanisms assessed by Tandem Mass Spectrometry.Journal of applied oral science : revista FOB · 2025Article
- In vitro reduction of enamel erosion by sugarcane-derived cystatin associated with sodium trimetaphosphate.Brazilian oral research · 2024Article
- Article
- Salivary protein candidates for biomarkers of oral disorders in people with a crack cocaine use disorder.Journal of applied oral science : revista FOB · 2023Article
- Salivary proteomic profile of young healthy subjects.Frontiers in molecular biosciences · 2023Article
- Label-Free Quantitative Proteomic Analysis Reveals Inflammatory Pattern Associated with Obesity and Periodontitis in Pregnant Women.Metabolites · 2022Article
- Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during, and after treatment.Clinical oral investigations · 2022Article
- Clinical Proteomics of Biofluids in Haematological Malignancies.International journal of molecular sciences · 2021Review
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- SWATH-MS Protocols in Human Diseases.Methods in molecular biology (Clifton, N.J.) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionSaliva contains numerous proteins and peptides, each of them carries a number of biological functions that are very important in maintaining the oral cavity health and also yields information about both local and systemic diseases. Currently, proteomic analysis is the basis for large-scale identification of these proteins and discovery of new biomarkers for distinct diseases.
objectiveThis study compared methodologies to extract salivary proteins for proteomic analysis. MATERIAL AND
methodsSaliva samples were collected from 10 healthy volunteers. In the first test, the necessity for using an albumin and IgG depletion column was evaluated, employing pooled samples from the 10 volunteers. In the second test, the analysis of the pooled samples was compared with individual analysis of one sample. Salivary proteins were extracted and processed for analysis by LC-ESI-MS/MS.
resultsIn the first test, we identified only 35 proteins using the albumin and IgG depletion column, while we identified 248 proteins without using the column. In the second test, the pooled sample identified 212 proteins, such as carbonic anhydrase 6, cystatin isoforms, histatins 1 and 3, lysozyme C, mucin 7, protein S100A8 and S100A9, and statherin, while individual analysis identified 239 proteins, among which are carbonic anhydrase 6, cystatin isoforms, histatin 1 and 3, lactotransferrin, lyzozyme C, mucin 7, protein S100A8 and S100A9, serotransferrin, and statherin.
conclusionsThe standardization of protocol for salivary proteomic analysis was satisfactory, since the identification detected typical salivary proteins, among others. The results indicate that using the column for depletion of albumin and IgG is not necessary and that performing individual analysis of saliva samples is possible.
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