ArticleMicrobiologyOpen2019
Harnessing microfluidic streak plate technique to investigate the gut microbiome of Reticulitermes chinensis.
Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Review
- Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application.Biosensors · 2025Review
- Advances in the isolation, cultivation, and identification of gut microbes.Military Medical Research · 2024Review
- Enrichment of different taxa of the enigmatic candidate phyla radiation bacteria using a novel picolitre droplet technique.ISME communications · 2024Article
- Research progress in isolation and identification of rumen probiotics.Frontiers in cellular and infection microbiology · 2024Review
- [Application of Droplet-Based Microfluidics in Microbial Research].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023Review
- Reaching unreachables: Obstacles and successes of microbial cultivation and their reasons.Frontiers in microbiology · 2023Review
- Emerging microfluidic technologies for microbiome research.Frontiers in microbiology · 2022Review
- One cell at a time: droplet-based microbial cultivation, screening and sequencing.Marine life science & technology · 2021Review
- Micro-Technologies for Assessing Microbial Dynamics in Controlled Environments.Frontiers in microbiology · 2021Review
- Bacteria Cultivated From Sponges and Bacteria Not Yet Cultivated From Sponges-A Review.Frontiers in microbiology · 2021Review
- What can microfluidics do for human microbiome research?Biomicrofluidics · 2020Article
- Culturomics of the plant prokaryotic microbiome and the dawn of plant-based culture media - A review.Journal of advanced research · 2019Review
- Harnessing microfluidic streak plate technique to investigate the gut microbiome of Reticulitermes chinensis.MicrobiologyOpen · 2019Article
- Diversity of Bacterial Biota inPathogens (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The termite gut microbiome is a model system to investigate microbial interactions and their associations with host. For decades, extensive research with molecular tools and conventional cultivation method has been carried out to define the microbial diversity in termite gut. Yet, many bacterial groups of the termite gut microbiome have not been successfully cultivated in laboratory. In this study, we adapted the recently developed microfluidic streak plate (MSP) technique for cultivation of termite gut microbial communities at both aerobic and anaerobic conditions. We found that 99 operational taxonomic units (OTUs) were cultivable by MSP approach and 18 OTUs were documented first time for termite gut microbiota. Further analysis of the bacterial diversities derived by culture-dependent MSP approach and culture-independent 16S rRNA gene typing revealed that both methods have bias in recovery of gut microbiota. In total 396 strains were isolated with MSP technique, and potential new taxa at species and/or genus levels were obtained that were phylogenetically related to Burkholderia, Micrococcus, and Dysgonomonas. Results from this study indicate that MSP technique is applicable for cultivating previously unknown and new microbial groups of termite gut microbiota.
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