ArticleThe Journal of general virology2018
Point-of-care diagnostic assay for the detection of Zika virus using the recombinase polymerase amplification method.
Article in The Journal of general virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Diagnostic accuracy of recombinase polymerase amplification-based assays for mosquito-borne virus detection in clinical samples: a systematic review and meta-analysis.Frontiers in cellular and infection microbiology · 2026Pooled it
- A need to raise the bar - A systematic review of temporal trends in diagnostics for Japanese encephalitis virus infection, and perspectives for future research.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2020Pooled it
- Amplification of RNA for identification of Zika and HCV in whole blood.Science advances · 2026Article
- Ten years of Zika in Brazil: achievements, challenges and perspectives.Virology journal · 2026Review
- Evolving Landscape of Emerging Virus Diagnosis: Challenges and Innovations.Molecular biotechnology · 2026Review
- Validation and Improvement of a Rapid, CRISPR-Cas-Free RPA-PCRD Strip Assay for On-Site Genomic Surveillance and Quarantine of Wheat Blast.Journal of fungi (Basel, Switzerland) · 2026Article
- The emergence of oropouche fever: A potential new threat?New microbes and new infections · 2025Article
- Development of an RT-RPA assay for La Crosse virus detection provides insights into age-dependent neuroinvasion in mice.Virology journal · 2025Article
- Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.ACS synthetic biology · 2025Article
- RT-RPA as a dual tool for detection and phylogenetic analysis of epidemic arthritogenic alphaviruses.Scientific reports · 2024Article
- Y-switch: a spring-loaded synthetic gene switch for robust DNA/RNA signal amplification and detection.Nucleic acids research · 2024Article
- Rapid detection of human adenovirus subgroup B using recombinase polymerase amplification assay.Virus genes · 2024Article
- Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives.Chemical science · 2023Review
- Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods.Frontiers in molecular biosciences · 2021Review
- Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases.Biosensors & bioelectronics · 2020Review
- A novel recombinase polymerase amplification assay for rapid detection of epidemic fowl adenovirus.Poultry science · 2020Article
- A flexible format LAMP assay for rapid detection of Ebola virus.PLoS neglected tropical diseases · 2020Article
- A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection.Micromachines · 2020Article
- Next-Generation Molecular Diagnostics Development by CRISPR/Cas Tool: Rapid Detection and Surveillance of Viral Disease Outbreaks.Frontiers in molecular biosciences · 2020Review
- Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.PloS one · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sudden and explosive expansion of Zika virus (ZIKV) from the African continent through Oceania and culminating in the outbreak in South America has highlighted the importance of new rapid point-of-care diagnostic tools for the control and prevention of transmission. ZIKV infection has devastating consequences, such as neurological congenital malformations in infants born to infected mothers and Guillain-Barré syndrome in adults. Additionally, its potential for transmission through vector bites, as well as from person to person through blood transfusions and sexual contact, are important considerations for prompt diagnosis. Recombinase polymerase amplification (RPA), an isothermal method, was developed as an alternative field-applicable assay to PCR. Here we report the development of a novel ZIKV real-time reverse transcriptase RPA (RT-RPA) assay capable of detecting a range of different ZIKV strains from a variety of geographical locations. The ZIKV RT-RPA was shown to be highly sensitive, being capable of detecting as few as five copies of target nucleic acid per reaction, and suitable for use with a battery-operated portable device. The ZIKV RT-RPA demonstrated 100 % specificity and 83 % sensitivity in clinical samples. Furthermore, we determined that the ZIKV RT-RPA is a versatile assay that can be applied to crude samples, such as saliva and serum, and can be used as a vector surveillance tool on crude mosquito homogenates. Therefore, the developed ZIKV RT-RPA is a useful diagnostic tool that can be transferred to a resource-limited location, eliminating the need for a specialized and sophisticated laboratory environment and highly trained staff.
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