ArticleAging and disease2018
CLARITY for High-resolution Imaging and Quantification of Vasculature in the Whole Mouse Brain.
Article in Aging and disease, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- A spatiotemporal atlas of cerebrovascular development in zebrafish.Nature communications · 2026Article
- Deep learning-based vessel extraction in 3D confocal microscope images of cleared human glioma tissues.Biomedical optics express · 2024Article
- A versatile vessel casting method for fine mapping of vascular networks using a hydrogel-based lipophilic dye solution.Cell reports methods · 2023Article
- Pathological changes within the cerebral vasculature in Alzheimer's disease: New perspectives.Brain pathology (Zurich, Switzerland) · 2022Review
- Optimal combinations of fluorescent vessel labeling and tissue clearing methods for three-dimensional visualization of vasculature.Neurophotonics · 2022Article
- Quantification of hypoxic regions distant from occlusions in cerebral penetrating arteriole trees.PLoS computational biology · 2022Article
- Advances in studying whole mouse brain vasculature using high-resolution 3D light microscopy imaging.Neurophotonics · 2022Review
- Brain virtual histology with X-ray phase-contrast tomography Part I: whole-brain myelin mapping in white-matter injury models.Biomedical optics express · 2022Article
- Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke.International journal of biological sciences · 2022Review
- Article
- Brain microvasculature has a common topology with local differences in geometry that match metabolic load.Neuron · 2021Article
- From arteries to capillaries: approaches to engineering human vasculature.Advanced functional materials · 2020Article
- MACS: Rapid Aqueous Clearing System for 3D Mapping of Intact Organs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Clearing techniques for visualizing the nervous system in development, injury, and disease.Journal of neuroscience methods · 2020Review
- Detection of Cerebrovascular Loss in the Normal Aging C57BL/6 Mouse Brain UsingFrontiers in aging neuroscience · 2020Article
- A Practical Guide to the Automated Analysis of Vascular Growth, Maturation and Injury in the Brain.Frontiers in neuroscience · 2020Article
- Microglia exacerbate white matter injury via complement C3/C3aR pathway after hypoperfusion.Theranostics · 2020Article
- The cerebral angiome: High resolution MicroCT imaging of the whole brain cerebrovasculature in female and male mice.NeuroImage · 2019Article
- A Simple Technique for Three-Dimensional Imaging and Segmentation of Brain Vasculature U sing Fast Free-of-Acrylamide Clearing Tissue in Murine.Cell journal · 2019Article
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Authors and funding
13 authors at 5 institutions in 3 countries.
Funding
Abstract
Elucidating the normal structure and distribution of cerebral vascular system is fundamental for understanding its function. However, studies on visualization and whole-brain quantification of vasculature with cellular resolution are limited. Here, we explored the structure of vasculature at the whole-brain level using the newly developed CLARITY technique. Adult male C57BL/6J mice undergoing transient middle cerebral artery occlusion and Tie2-RFP transgenic mice were used. Whole mouse brains were extracted for CLARITY processing. Immunostaining was performed to label vessels. Customized MATLAB code was used for image processing and quantification. Three-dimensional images were visualized using the Vaa3D software. Our results showed that whole mouse brain became transparent using the CLARITY method. Three-dimensional imaging and visualization of vasculature were achieved at the whole-brain level with a 1-μm voxel resolution. The quantitative results showed that the fractional vascular volume was 0.018 ± 0.004 mm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.