ArticleInternational journal of oral science2018
Bone-conditioned medium contributes to initiation and progression of osteogenesis by exhibiting synergistic TGF-β1/BMP-2 activity.
Article in International journal of oral science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Total flavonoids of Rhizoma Drynariae promote osteogenic differentiation of tendon-derived stem cells through the TGF-β/BMP signaling pathway, thereby improving bone regeneration after anterior cruciate ligament reconstruction in rats.In vitro cellular & developmental biology. Animal · 2026Article
- Using an Incidental Separated Segment of the Socket Wall as an Autogenous Block: A Lesson Learned Case Report of Three-Dimensional Bone Reconstruction in the Anterior Maxilla.Clinical case reports · 2026Article
- 3D printed scaffolds regulated by neural-bone metabolic coupling promote bone unit regeneration.Bioactive materials · 2026Article
- TGF-β/BMP signaling in skeletal biology: molecular mechanisms, regulatory networks, and therapeutic implications in development, regeneration, and disease.Bone research · 2026Review
- Vertical Ridge Augmentation in the Anterior Maxilla Using a Dual-Texture e-PTFE Membrane and Autogenous Bone: A 2- to 5-Year Retrospective Case-Series Study.Clinical oral implants research · 2025Article
- Histological study of the effect of different hydration times of bone allograft and xenograft particles on the rate of bone formation in critical size defects in the rat calvarium.International journal of implant dentistry · 2025Article
- Review
- Biofunctionalization of Collagen Barrier Membranes with Bone-Conditioned Medium, as a Natural Source of Growth Factors, Enhances Osteoblastic Cell Behavior.International journal of molecular sciences · 2025Article
- Research progress of gene therapy combined with tissue engineering to promote bone regeneration.APL bioengineering · 2024Review
- Differential Gene Expression Involved in Bone Turnover of Mice Expressing Constitutively Active TGFβ Receptor Type I.International journal of molecular sciences · 2024Article
- The effects of heat and hydrogen peroxide treatment on the osteoinductivity of demineralized cortical bone: a potential method for preparing tendon/ligament repair scaffolds.Regenerative biomaterials · 2024Article
- Enhancing Mechanical and Biological Properties of Zinc Phosphate Dental Cement with Akermanite and Hardystonite Nanoparticles: A Synthesis and Characterization Study.International journal of dentistry · 2024Article
- Differential molecular profiles and associated functionalities characterize connective tissue grafts obtained at different locations and depths in the human palate.International journal of oral science · 2023Article
- Local Concentrations of TGF-β1 and IGF-1 Appear Determinant in Regulating Bone Regeneration in Human Postextraction Tooth Sockets.International journal of molecular sciences · 2023Article
- Article
- A programmed surface on polyetheretherketone for sequentially dictating osteoimmunomodulation and bone regeneration to achieve ameliorative osseointegration under osteoporotic conditions.Bioactive materials · 2022Article
- miR-150-5p inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by targeting VDR.Experimental and therapeutic medicine · 2022Article
- Primary cilia: The central role in the electromagnetic field induced bone healing.Frontiers in pharmacology · 2022Review
- Mechanical Properties of Human Concentrated Growth Factor (CGF) Membrane and the CGF Graft with Bone Morphogenetic Protein-2 (BMP-2) onto Periosteum of the Skull of Nude Mice.International journal of molecular sciences · 2021Article
- Regulation of extracellular bioactive cations in bone tissue microenvironment induces favorable osteoimmune conditions to accelerateBioactive materials · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral (DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P < 0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2 was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.