Evidence map›Paper›PMID 29895743›Full record

ArticleInternational journal of molecular sciences2018

Berberine Protects Human Retinal Pigment Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Damage through Activation of AMPK.

Shuai Li, Uma Gaur, Cheong-Meng Chong, Shaofen Lin, Jiankang Fang, Zhiwen Zeng, Haitao Wang, Wenhua Zheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Mitochondrial Resilience in Glaucoma: Targeting NADDiseases (Basel, Switzerland) · 2026
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  17. Ferulic acid (FA) protects human retinal pigment epithelial cells from HJournal of cellular and molecular medicine · 2020
    Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shuai LiFaculty of Health Science, University of Macau, Taipa, Macau 999078, China. yb67619@umac.mo.
Uma GaurFaculty of Health Science, University of Macau, Taipa, Macau 999078, China. gaur.uma2906@gmail.com.
Cheong-Meng ChongFaculty of Health Science, University of Macau, Taipa, Macau 999078, China. legendhero91@gmail.com.
Shaofen LinState Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center and School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510000, China. linshaofen1970@163.com.
Jiankang FangFaculty of Health Science, University of Macau, Taipa, Macau 999078, China. yb57646@umac.mo.
Zhiwen ZengFaculty of Health Science, University of Macau, Taipa, Macau 999078, China. zengzw1122@gmail.com.
Haitao WangDepartment of Neuropharmacology and Novel Drug Discovery, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510000, China. wht821@smu.edu.cn.
Wenhua ZhengFaculty of Health Science, University of Macau, Taipa, Macau 999078, China. wenhuazheng@umac.mo.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Age-related macular degeneration (AMD) is the leading cause of central vision loss in the elderly with less effective treatment, especially for dry AMD (90% of AMD). Although the etiology of this disease is not well elucidated, increasing evidences indicate that excessive reactive oxygen species (ROS) impairing the physiological functions of retinal pigment epithelium (RPE) cells may be one of the main causes. Therefore, it could be a great strategy to find some drugs that can effectively protect RPE cells from oxidative damage which is desired to treat and slow the process of AMD. In the present study, a well-known traditional Chinese medicine berberine (BBR) was found to suppress hydrogen peroxide (H₂O₂)-induced oxidative damage in D407 cells, a human RPE cell line. Pre-treatment of D407 cells with BBR significantly suppressed H₂O₂-induced cell apoptosis by restoring abnormal changes in nuclear morphology, preventing the decline of mitochondrial membrane potential, reducing lactate dehydrogenase release and inhibiting caspase 3/7 activities induced by H₂O₂. Western blot analysis showed that BBR was able to stimulate the phosphorylation/activation of AMPK in a time- and dose-dependent manner in D407 cells, while treatment of cells with AMPK pathway inhibitor Compound C, or knockdown of the AMPK by specific siRNA blocked the effect of BBR. Similar results were obtained in primary cultured human RPE cells. Taken together, these results demonstrated that BBR was able to protect RPE cells against oxidative stress via the activation of AMPK pathway. Our findings also indicate the potential application of BBR in AMD treatment.

Indexed as

AMP-Activated Protein KinasesApoptosisBerberineCell LineHumansHydrogen PeroxideMembrane Potential, MitochondrialOxidation-ReductionOxidative StressReactive Oxygen SpeciesRetinal Pigment EpitheliumSignal TransductionAMP-Activated Protein KinasesBerberineHydrogen PeroxideReactive Oxygen Speciesage-related macular degenerationAMPKberberineD407 cells

Identifiers

PMID29895743
PMCPMC6032421

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.