ArticleProceedings of the National Academy of Sciences of the United States of America2018
Microfluidic chambers using fluid walls for cell biology.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Generation of Clonal Cultures of Adherent or Suspension Cells Using Flat Sessile Drops for Assurance of Monoclonality.Biotechnology and bioengineering · 2025Article
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.ACS biomaterials science & engineering · 2025Article
- Single-Cell Lipidomics: An Automated and Accessible Microfluidic Workflow Validated by Capillary Sampling.Analytical chemistry · 2024Article
- Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.bioRxiv : the preprint server for biology · 2024Article
- Adaptive robustness through incoherent signaling mechanisms in a regenerative brain.Cell reports · 2024Article
- Automated and miniaturized screening of antibiotic combinationsActa pharmaceutica Sinica. B · 2024Article
- Visualization-enhanced under-oil open microfluidic system for in situ characterization of multi-phase chemical reactions.Nature communications · 2024Article
- Magnetic Janus origami robot for cross-scale droplet omni-manipulation.Nature communications · 2023Article
- Microfluidics engineering towards personalized oncology-a review.In vitro models · 2023Review
- Machining water through laser cutting of nanoparticle-encased water pancakes.Nature communications · 2023Article
- Miniaturizing chemistry and biology using droplets in open systems.Nature reviews. Chemistry · 2023Review
- A platform for modular assembly and feeding of micro-organoids on standard Petri dishes.Biology open · 2023Article
- Under-oil open microfluidic systems for rapid phenotypic antimicrobial susceptibility testing.Lab on a chip · 2023Article
- Interfacial tension driven open droplet microfluidics.Advanced materials interfaces · 2023Article
- A microfluidic perspective on conventional in vitro transcranial direct current stimulation methods.Journal of neuroscience methods · 2023Review
- Reconfigurable Microfluidic Circuits for Isolating and Retrieving Cells of Interest.ACS applied materials & interfaces · 2022Article
- Under-Oil Autonomously Regulated Oxygen Microenvironments: A Goldilocks Principle-Based Approach for Microscale Cell Culture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Instability of a liquid sheet with viscosity contrast in inertial microfluidics.The European physical journal. E, Soft matter · 2021Article
- Predicting flows through microfluidic circuits with fluid walls.Microsystems & nanoengineering · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Many proofs of concept have demonstrated the potential of microfluidics in cell biology. However, the technology remains inaccessible to many biologists, as it often requires complex manufacturing facilities (such as soft lithography) and uses materials foreign to cell biology (such as polydimethylsiloxane). Here, we present a method for creating microfluidic environments by simply reshaping fluids on a substrate. For applications in cell biology, we use cell media on a virgin Petri dish overlaid with an immiscible fluorocarbon. A hydrophobic/fluorophilic stylus then reshapes the media into any pattern by creating liquid walls of fluorocarbon. Microfluidic arrangements suitable for cell culture are made in minutes using materials familiar to biologists. The versatility of the method is demonstrated by creating analogs of a common platform in cell biology, the microtiter plate. Using this vehicle, we demonstrate many manipulations required for cell culture and downstream analysis, including feeding, replating, cloning, cryopreservation, lysis plus RT-PCR, transfection plus genome editing, and fixation plus immunolabeling (when fluid walls are reconfigured during use). We also show that mammalian cells grow and respond to stimuli normally, and worm eggs develop into adults. This simple approach provides biologists with an entrée into microfluidics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.