ArticlePloS one2018
Comprehensive analysis of Verticillium nonalfalfae in silico secretome uncovers putative effector proteins expressed during hop invasion.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- A protein elicitor PeVn1 fromFrontiers in plant science · 2024Article
- Novel Identification of the Collection of Pathogenic Fungal SpeciesPathogens (Basel, Switzerland) · 2023Article
- Discovery of microRNA-like Small RNAs in Pathogenic Plant FungusInternational journal of molecular sciences · 2022Article
- Infection Process and Genome Assembly Provide Insights into the Pathogenic Mechanism of Destructive MycoparasiteJournal of fungi (Basel, Switzerland) · 2021Article
- Unraveling the Roles of Vascular Proteins Using Proteomics.Molecules (Basel, Switzerland) · 2021Review
- Hop Polyphenols in Relation to Verticillium Wilt Resistance and Their Antifungal Activity.Plants (Basel, Switzerland) · 2020Article
- EffHunter: A Tool for Prediction of Effector Protein Candidates in Fungal Proteomic Databases.Biomolecules · 2020Article
- In silico approach to predict pancreatic β-cells classically secreted proteins.Bioscience reports · 2020Article
- Article
- Time-Course RNAseq RevealsFrontiers in microbiology · 2020Article
- RNA interference core components identified and characterised in Verticillium nonalfalfae, a vascular wilt pathogenic plant fungi of hops.Scientific reports · 2019Article
- Flax rust infection transcriptomics reveals a transcriptional profile that may be indicative for rust Avr genes.PloS one · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vascular plant pathogen Verticillium nonalfalfae causes Verticillium wilt in several important crops. VnaSSP4.2 was recently discovered as a V. nonalfalfae virulence effector protein in the xylem sap of infected hop. Here, we expanded our search for candidate secreted effector proteins (CSEPs) in the V. nonalfalfae predicted secretome using a bioinformatic pipeline built on V. nonalfalfae genome data, RNA-Seq and proteomic studies of the interaction with hop. The secretome, rich in carbohydrate active enzymes, proteases, redox proteins and proteins involved in secondary metabolism, cellular processing and signaling, includes 263 CSEPs. Several homologs of known fungal effectors (LysM, NLPs, Hce2, Cerato-platanins, Cyanovirin-N lectins, hydrophobins and CFEM domain containing proteins) and avirulence determinants in the PHI database (Avr-Pita1 and MgSM1) were found. The majority of CSEPs were non-annotated and were narrowed down to 44 top priority candidates based on their likelihood of being effectors. These were examined by spatio-temporal gene expression profiling of infected hop. Among the highest in planta expressed CSEPs, five deletion mutants were tested in pathogenicity assays. A deletion mutant of VnaUn.279, a lethal pathotype specific gene with sequence similarity to SAM-dependent methyltransferase (LaeA), had lower infectivity and showed highly reduced virulence, but no changes in morphology, fungal growth or conidiation were observed. Several putative secreted effector proteins that probably contribute to V. nonalfalfae colonization of hop were identified in this study. Among them, LaeA gene homolog was found to act as a potential novel virulence effector of V. nonalfalfae. The combined results will serve for future characterization of V. nonalfalfae effectors, which will advance our understanding of Verticillium wilt disease.
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