ArticleLaboratory medicine2018
Characterization of Storage-Induced Red Blood Cell Hemolysis Using Raman Spectroscopy.
Article in Laboratory medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Advancing Transfusion Medicine through Raman Tweezers Spectroscopy: A Review of Recent Progress and Future Perspectives.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2024Review
- Influence of storage and buffer composition on the mechanical behavior of flowing red blood cells.Biophysical journal · 2023Article
- Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka.African journal of laboratory medicine · 2023Article
- Fiber optic Raman spectroscopy for the evaluation of disease state in Duchenne muscular dystrophy: An assessment using the mdx model and human muscle.Muscle & nerve · 2022Article
- HBOC-301 in Porcine Kidney Normothermic Machine Perfusion and the Effect of Vitamin C on Methemoglobin Formation.Antioxidants (Basel, Switzerland) · 2022Article
- Article
- Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections.Frontiers in medicine · 2022Article
- Do We Store Packed Red Blood Cells under "Quasi-Diabetic" Conditions?Biomolecules · 2021Review
- Biophotonic probes for bio-detection and imaging.Light, science & applications · 2021Review
- Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation: A Mini-Review.Frontiers in physiology · 2021Review
- Diagnosing sickle cell disease and iron deficiency anemia in human blood by Raman spectroscopy.Lasers in medical science · 2020Article
- Micro-Raman Spectroscopy Analysis of Optically Trapped Erythrocytes in Jaundice.Frontiers in physiology · 2020Article
- Optofluidic laser speckle image decorrelation analysis for the assessment of red blood cell storage.PloS one · 2019Article
- Optical force-induced nonlinearity and self-guiding of light in human red blood cell suspensions.Light, science & applications · 2019Article
- Non-invasive analysis of stored red blood cells using diffuse resonance Raman spectroscopy.The Analyst · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. The accuracy of current methods of measuring hemolysis can suffer as a result of specimen collection and processing artefacts.
objectiveTo test whether Raman spectroscopy could be used to assess hemolysis.
methodsRBCs were stored for as long as 42 days. Raman spectra of RBCs were measured before and after washing, and hemolysis was measured in supernatant by visible spectroscopy.
resultsRaman spectra indicated increased concentrations of oxyhemoglobin (oxyHb) and methemoglobin (metHb), and decreased membrane fluidity with storage age. Changes in oxyHb and metHb were associated with the intraerythrocytic and extracellular fractions, respectively. Hemolysis increased in a storage age-dependent manner. Changes in Raman bands reflective of oxyHb, metHb, and RBC membranes correlated with hemolysis; the most statistically significant change was an increased intensity of metHb and decreased membrane fluidity.
conclusionsThese data suggest that Raman spectroscopy may offer a new label-free modality to assess RBC hemolysis during cold storage.
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