ArticleToxicological sciences : an official journal of the Society of Toxicology2019
Multi-Behavioral Endpoint Testing of an 87-Chemical Compound Library in Freshwater Planarians.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- Backward scrunching: planarians escape from noxious stimuli, cannibalism, and predation.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2026Article
- Timing of exposure impacts how organophosphorus pesticides affect the developing brain in the planarian Dugesia japonica.Archives of toxicology · 2026Article
- Planarian behavioral screening is a useful invertebrate model for evaluating seizurogenic chemicals.Neurotoxicology · 2026Article
- High-Throughput Toxicity Screening withEnvironmental science & technology · 2026Review
- Rapid behavioral screening in the planarianFrontiers in toxicology · 2026Article
- High-content toxicological profiling of 87 compounds using a 3D mouse mini-testis model: a New Approach Methodology (NAM) for prioritizing male reproductive toxicants.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Red Midge Larvae Are an Invertebrate Alternative Diet to Beef Liver for Planarian Husbandry.Biomolecules · 2025Article
- Statistical analysis of multi-endpoint phenotypic screening increases sensitivity of planarian neurotoxicity testing.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Planarian behavioral screening is a useful invertebrate model for evaluating seizurogenic chemicals.bioRxiv : the preprint server for biology · 2025Article
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- How neurobehavior and brain development in alternative whole-organism models can contribute to prediction of developmental neurotoxicity.Neurotoxicology · 2024Review
- Modified polymer membranes for the removal of pharmaceutical active compounds in wastewater and its mechanism-A review.Bioengineered · 2023Review
- Comparative toxicity assessment of glyphosate and two commercial formulations in the planarianFrontiers in toxicology · 2023Article
- New Worm on the Block: Planarians in (Neuro)Toxicology.Current protocols · 2022Article
- Bioactivation and detoxification of organophosphorus pesticides in freshwater planarians shares similarities with humans.Archives of toxicology · 2022Article
- Head removal enhances planarian electrotaxis.The Journal of experimental biology · 2022Article
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- Sensory-Motor Perturbations in Larval Zebrafish (International journal of molecular sciences · 2022Article
- Current status and future directions for a neurotoxicity hazard assessment framework that integratesComputational toxicology (Amsterdam, Netherlands) · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
There is an increased recognition in the field of toxicology of the value of medium-to-high-throughput screening methods using in vitro and alternative animal models. We have previously introduced the asexual freshwater planarian Dugesia japonica as a new alternative animal model and proposed that it is particularly well-suited for the study of developmental neurotoxicology. In this article, we discuss how we have expanded and automated our screening methodology to allow for fast screening of multiple behavioral endpoints, developmental toxicity, and mortality. Using an 87-compound library provided by the National Toxicology Program, consisting of known and suspected neurotoxicants, including drugs, flame retardants, industrial chemicals, polycyclic aromatic hydrocarbons (PAHs), pesticides, and presumptive negative controls, we further evaluate the benefits and limitations of the system for medium-throughput screening, focusing on the technical aspects of the system. We show that, in the context of this library, planarians are the most sensitive to pesticides with 16/16 compounds causing toxicity and the least sensitive to PAHs, with only 5/17 causing toxicity. Furthermore, while none of the presumptive negative controls were bioactive in adult planarians, 2/5, acetaminophen and acetylsalicylic acid, were bioactive in regenerating worms. Notably, these compounds were previously reported as developmentally toxic in mammalian studies. Through parallel screening of adults and developing animals, planarians are thus a useful model to detect such developmental-specific effects, which was observed for 13 chemicals in this library. We use the data and experience gained from this screen to propose guidelines for best practices when using planarians for toxicology screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.