ArticleGigaScience2018
Improved de novo genome assembly and analysis of the Chinese cucurbit Siraitia grosvenorii, also known as monk fruit or luo-han-guo.
Article in GigaScience, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Article
- Haplotype-resolved T2T genome and population resequencing provide insights into the domestication and mogroside biosynthesis ofHorticulture research · 2026Article
- Annotating genomes at increased scale and resolution.Nature reviews. Genetics · 2026Review
- Comparative analysis of the Aquaporin gene family in Cucurbitaceae: insights into evolutionary patterns and functional diversity.BMC plant biology · 2026Article
- OMAnnotator: a novel approach to building an annotated consensus genome sequence.Bioinformatics advances · 2026Article
- A review ofChinese herbal medicines · 2026Review
- Phylogeographic analysis ofEcology and evolution · 2023Article
- Lateral Root Initiation in Cucumber (International journal of molecular sciences · 2023Article
- CuGenDBv2: an updated database for cucurbit genomics.Nucleic acids research · 2023Article
- The Current Developments in Medicinal Plant Genomics Enabled the Diversification of Secondary Metabolites' Biosynthesis.International journal of molecular sciences · 2022Review
- Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides.International journal of molecular sciences · 2022Article
- Impact of LTR-Retrotransposons on Genome Structure, Evolution, and Function in Curcurbitaceae Species.International journal of molecular sciences · 2022Article
- Natural products of medicinal plants: biosynthesis and bioengineering in post-genomic era.Horticulture research · 2022Article
- A Combined Transcriptomic and Proteomic Approach to Reveal the Effect of Mogroside V on OVA-Induced Pulmonary Inflammation in Mice.Frontiers in immunology · 2022Article
- Application of High-Throughput Sequencing on the Chinese Herbal Medicine for the Data-Mining of the Bioactive Compounds.Frontiers in plant science · 2022Review
- Chromosome-level genome assembly of Gynostemma pentaphyllum provides insights into gypenoside biosynthesis.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- The first draft genome of Picrorhiza kurrooa, an endangered medicinal herb from Himalayas.Scientific reports · 2021Article
- Introduction, adaptation and characterization of monk fruit (Siraitia grosvenorii): a non-caloric new natural sweetener.Scientific reports · 2021Article
- Network Pharmacology and Inflammatory Microenvironment Strategy Approach to Finding the Potential Target ofFrontiers in genetics · 2021Article
- Review on the Development and Applications of Medicinal Plant Genomes.Frontiers in plant science · 2021Review
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Authors and funding
8 authors.
Funding
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Abstract
Background: Luo-han-guo (Siraitia grosvenorii), also called monk fruit, is a member of the Cucurbitaceae family. Monk fruit has become an important area for research because of the pharmacological and economic potential of its noncaloric, extremely sweet components (mogrosides). It is also commonly used in traditional Chinese medicine for the treatment of lung congestion, sore throat, and constipation. Recently, a single reference genome became available for monk fruit, assembled from 36.9x genome coverage reads via Illumina sequencing platforms. This genome assembly has a relatively short (34.2 kb) contig N50 length and lacks integrated annotations. These drawbacks make it difficult to use as a reference in assembling transcriptomes and discovering novel functional genes. Findings: Here, we offer a new high-quality draft of the S. grosvenorii genome assembled using 31 Gb (∼73.8x) long single molecule real time sequencing reads and polished with ∼50 Gb Illumina paired-end reads. The final genome assembly is approximately 469.5 Mb, with a contig N50 length of 432,384 bp, representing a 12.6-fold improvement. We further annotated 237.3 Mb of repetitive sequence and 30,565 consensus protein coding genes with combined evidence. Phylogenetic analysis showed that S. grosvenorii diverged from members of the Cucurbitaceae family approximately 40.9 million years ago. With comprehensive transcriptomic analysis and differential expression testing, we identified 4,606 up-regulated genes in the early fruit compared to the leaf, a number of which were linked to metabolic pathways regulating fruit development and ripening. Conclusions: The availability of this new monk fruit genome assembly, as well as the annotations, will facilitate the discovery of new functional genes and the genetic improvement of monk fruit.
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