ArticleBiology of reproduction2018
Intracellular Ca2+ threshold reversibly switches flagellar beat off and on.
Article in Biology of reproduction, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Increased reproductive outcomes after optimized sperm preparation.Frontiers in cell and developmental biology · 2025Article
- The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis.The Journal of cell biology · 2024Article
- CaAnimal cells and systems · 2024Review
- Analysis of CaFrontiers in cell and developmental biology · 2024Article
- Effects of preconception exposure to phthalates on mouse sperm capacitation parameters.Andrology · 2023Article
- Elevated and Sustained Intracellular Calcium Signalling Is Necessary for Efficacious Induction of the Human Sperm Acrosome Reaction.International journal of molecular sciences · 2022Article
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- The interaction between free CaParasitology research · 2022Article
- C2cd6-encoded CatSperτ targets sperm calcium channel to CaCell reports · 2022Article
- Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency.Biology · 2021Article
- Article
- Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Structure and Function of Ion Channels Regulating Sperm Motility-An Overview.International journal of molecular sciences · 2021Review
- Modelling Motility: The Mathematics of Spermatozoa.Frontiers in cell and developmental biology · 2021Review
- Capacitation increases glucose consumption in murine sperm.Molecular reproduction and development · 2020Article
- Differences and Similarities: The Richness of Comparative Sperm Physiology.Physiology (Bethesda, Md.) · 2020Review
- Continuous behavioural 'switching' in human spermatozoa and its regulation by Ca2+-mobilising stimuli.Molecular human reproduction · 2019Article
- Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies.Frontiers in cell and developmental biology · 2019Article
- Expression Analysis of the CRISP2, CATSPER1, PATE1 and SEMG1 in the Sperm of Men with Idiopathic Asthenozoospermia.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Sperm motility is essential for fertilization. The asymmetry of flagellar beat in spermatozoa is finely regulated by intracellular calcium concentration ([Ca2+]i). Recently, we demonstrated that the application of high concentrations (10-20 μM) of the Ca2+ ionophore A23187 promotes sperm immobilization after 10 min, and its removal thereafter allows motility recovery, hyperactivation, and fertilization. In addition, the same ionophore treatment overcomes infertility observed in sperm from Catsper1-/-, Slo3-/-, and Adcy10-/-, but not PMCA4-/-, which strongly suggest that regulation of [Ca2+]i is mandatory for sperm motility and hyperactivation. In this study, we found that prior to inducing sperm immobilization, high A23187 concentrations (10 μM) increase flagellar beat. While 5-10 μM A23187 substantially elevates [Ca2+]i and rapidly immobilizes sperm in a few minutes, smaller concentrations (0.5 and 1 μM) provoke smaller [Ca2+]i increases and sperm hyperactivation, confirming that [Ca2+]i increases act as a motility switch. Until now, the [Ca2+]i thresholds that switch motility on and off were not fully understood. To study the relationship between [Ca2+]i and flagellar beating, we developed an automatic tool that allows the simultaneous measurement of these two parameters. Individual spermatozoa were treated with A23187, which is then washed to evaluate [Ca2+]i and flagellar beat recovery using the implemented method. We observe that [Ca2+]i must decrease below a threshold concentration range to facilitate subsequent flagellar beat recovery and sperm motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.