ArticleThe Journal of cell biology1985
An efficient method for introducing macromolecules into living cells.
Article in The Journal of cell biology, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
51 citing papers in PubMed, 114 citations in OpenAlex.
- Aspects of Biological Replication and Evolution Independent of the Central Dogma: Insights from Protein-Free Vesicular Transformations and Protein-Mediated Membrane Remodeling.The Journal of membrane biology · 2022Article
- Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains.Scientific reports · 2016Article
- Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules.ACS nano · 2013Article
- IFITM proteins restrict viral membrane hemifusion.PLoS pathogens · 2013Article
- The final conformation of the complete ectodomain of the HA2 subunit of influenza hemagglutinin can by itself drive low pH-dependent fusion.The Journal of biological chemistry · 2011Article
- Dengue virus ensures its fusion in late endosomes using compartment-specific lipids.PLoS pathogens · 2010Article
- An adhesion-based method for plasma membrane isolation: evaluating cholesterol extraction from cells and their membranes.Analytical biochemistry · 2009Article
- Cytoskeleton reorganization in influenza hemagglutinin-initiated syncytium formation.Biochimica et biophysica acta · 2009Article
- Class II fusion protein of alphaviruses drives membrane fusion through the same pathway as class I proteins.The Journal of cell biology · 2005Article
- Reversible stages of the low-pH-triggered conformational change in influenza virus hemagglutinin.The EMBO journal · 2002Article
- Synchronized activation and refolding of influenza hemagglutinin in multimeric fusion machines.The Journal of cell biology · 2001Article
- Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Evolution of intermediates of influenza virus hemagglutinin-mediated fusion revealed by kinetic measurements of pore formation.Biophysical journal · 2001Article
- The anti-influenza virus agent 4-GU-DANA (zanamivir) inhibits cell fusion mediated by human parainfluenza virus and influenza virus HA.Journal of virology · 2000Article
- Reversible merger of membranes at the early stage of influenza hemagglutinin-mediated fusion.Molecular biology of the cell · 2000Article
- Hemifusion between cells expressing hemagglutinin of influenza virus and planar membranes can precede the formation of fusion pores that subsequently fully enlarge.Biophysical journal · 1999Article
- Tension of membranes expressing the hemagglutinin of influenza virus inhibits fusion.Biophysical journal · 1999Article
- Antigen-specific membrane fusion mediated by the haemagglutinin protein of influenza A virus: separation of attachment and fusion functions on different molecules.Gene therapy · 1999Article
- Microtubule-dependent plus- and minus end-directed motilities are competing processes for nuclear targeting of adenovirus.The Journal of cell biology · 1999Article
- Effects of spontaneous bilayer curvature on influenza virus-mediated fusion pores.The Journal of general physiology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The hemagglutinin (HA) of influenza virus was used to obtain efficient and rapid bulk delivery of antibodies and horseradish peroxidase (HRP) into the cytoplasm of living tissue culture cells. By exploiting HA's efficient cell surface expression, its high affinity for erythrocytes, and its acid-dependent membrane fusion activity, a novel delivery method was developed. The approach is unique in that the mediator of both binding and fusion (the HA) is present on the surfaces of the target cells. A recently developed 3T3 cell line which permanently expresses HA, Madin-Darby canine kidney cells infected with influenza virus, and CV-1 cells infected with a simian virus 40 vector carrying the HA gene were used as recipient cells. Protein-loaded erythrocytes were bound to the HA on the cell surface and a brief drop in pH to 5.0 was used to trigger HA's fusion activity and hence delivery. About 3 to 8 erythrocytes fused per 3T3 and CV-1 cell, respectively, and 75-95% of the cells received IgG or HRP. Quantitative analysis showed that 1.8 X 10(8) molecules of HRP and 1.4 X 10(7) IgG molecules were delivered per CV-1 cell and 6.2 X 10(7) HRP molecules per 3T3 cell. Cell viability, as judged by methionine incorporation into protein and cell growth and division, was not impaired. Electron and fluorescence microscopy showed that the fused erythrocyte membranes remained as discrete domains in the cell's plasma membrane. The method is simple, reliable, and nonlytic. The ability to simultaneously and rapidly deliver impermeable substances into large numbers of cells will permit biochemical analysis of the fate and effect of a variety of delivered molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.