Evidence map›Paper›PMID 29892942›Full record

ArticleGenes & genomics2018

Transcriptome-based identification of the optimal reference genes as internal controls for quantitative RT-PCR in razor clam (Sinonovacula constricta).

Xuelin Zhao, Jianping Fu, Liting Jiang, Weiwei Zhang, Yina Shao, Chunhua Jin, Jinbo Xiong, Chenghua Li

Abstract read
PubMed Publisher
In one paragraph

Article in Genes & genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
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  4. Signatures of selection inEvolutionary applications · 2024
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  5. Expression and Functional Analysis ofAnimals : an open access journal from MDPI · 2023
    Article
  6. Article
  7. Article
  8. Article
  9. Vital Role ofFrontiers in physiology · 2021
    Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Xuelin ZhaoSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Jianping FuSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Liting JiangSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Weiwei ZhangSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Yina ShaoSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Chunhua JinSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Jinbo XiongSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China.
Chenghua LiSchool of Marine Sciences, Ningbo University, 818 Fenghua Road, Ningbo, Zhejiang, 315211, People's Republic of China. lichenghua@nbu.edu.cn.
Ningbo University · CN

Funding

Natural Science Foundation of Ningbo 2015C10009Natural Science Foundation of Ningbo 2015C10062School Fund of Ningbo University XYL17010Zhejiang Major Program of Science and Technology 2015C32004Zhejiang Major Program of Science and Technology 2016C02055-9
6 · The paper itself

Abstract

Quantitative real-time PCR (qRT-PCR) is a standard method to measure gene expression in function exploring. Accurate and reproducible data of qRT-PCR requires appropriate reference genes, which are stably expressed under different experimental conditions. However, no housekeeping genes were validated as internal controls for qRT-PCR in Sinonovacula constricta. In this study, we classified the transcriptome data of two tissues for Vibrio infection and Cd

Indexed as

AnimalsBivalviaGene Expression ProfilingReal-Time Polymerase Chain ReactionReference StandardsReproducibility of ResultsTranscriptomeQuantitative RT-PCRReference genesSinonovacula constrictaTranscriptomeVibrio

Identifiers

PMID29892942
OpenAlexW2786919196

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.