ArticleLab on a chip2018
NemaFlex: a microfluidics-based technology for standardized measurement of muscular strength of C. elegans.
Article in Lab on a chip, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 46 citations in OpenAlex.
- Defining Microbiome Impact on Host Physiology During Spaceflight Using Caenorhabditis elegans.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A review: oxidative stress in skeletal muscle and the non-coding RNAs behind it.Molecular and cellular biochemistry · 2025Review
- Micropillars in Cell Mechanobiology: Design, Fabrication, Characterization, and Biosensing Applications.Small science · 2025Review
- FHOD-1 and profilin protect sarcomeres against contraction-induced deformation> inMolecular biology of the cell · 2024Article
- Protein kinase 2 of the giant sarcomeric protein UNC-89 regulates mitochondrial morphology and function.Communications biology · 2024Article
- FHOD-1/profilin-mediated actin assembly protects sarcomeres against contraction-induced deformation inbioRxiv : the preprint server for biology · 2024Article
- Lab-on-chip technologies for space research - current trends and prospects.Mikrochimica acta · 2023Review
- Spaceflight Induces Strength Decline inCells · 2023Article
- Mitochondrial sulfide promotes life span and health span through distinct mechanisms in developing versus adult treatedProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microfluidic techniques for mechanical measurements of biological samples.Biophysics reviews · 2023Review
- A Compact Imaging Platform for ConductingLife (Basel, Switzerland) · 2023Article
- Evolution of focused streams for viscoelastic flow in spiral microchannels.Microsystems & nanoengineering · 2023Article
- Microfluidics-integrated spaceflight hardware for measuring muscle strength of Caenorhabditis elegans on the International Space Station.NPJ microgravity · 2022Article
- Investigating the correlation of muscle function tests and sarcomere organization in C. elegans.Skeletal muscle · 2021Article
- Mitochondrial hydrogen sulfide supplementation improves health in theProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biophysical analysis of drug efficacy on C. elegans models for neurodegenerative and neuromuscular diseases.PloS one · 2021Article
- NemaLife chip: a micropillar-based microfluidic culture device optimized for aging studies in crawling C. elegans.Scientific reports · 2020Article
- A Region of UNC-89 (Obscurin) Lying between Two Protein Kinase Domains Is a Highly Elastic Spring Required for Proper Sarcomere Organization.Journal of molecular biology · 2020Article
- Article
- Tart Cherry Increases Lifespan inNutrients · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Muscle strength is a functional measure of quality of life in humans. Declines in muscle strength are manifested in diseases as well as during inactivity, aging, and space travel. With conserved muscle biology, the simple genetic model C. elegans is a high throughput platform in which to identify molecular mechanisms causing muscle strength loss and to develop interventions based on diet, exercise, and drugs. In the clinic, standardized strength measures are essential to quantitate changes in patients; however, analogous standards have not been recapitulated in the C. elegans model since force generation fluctuates based on animal behavior and locomotion. Here, we report a microfluidics-based system for strength measurement that we call 'NemaFlex', based on pillar deflection as the nematode crawls through a forest of pillars. We have optimized the micropillar forest design and identified robust measurement conditions that yield a measure of strength that is independent of behavior and gait. Validation studies using a muscle contracting agent and mutants confirm that NemaFlex can reliably score muscular strength in C. elegans. Additionally, we report a scaling factor to account for animal size that is consistent with a biomechanics model and enables comparative strength studies of mutants. Taken together, our findings anchor NemaFlex for applications in genetic and drug screens, for defining molecular and cellular circuits of neuromuscular function, and for dissection of degenerative processes in disuse, aging, and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.