ArticleNature chemistry2018
Infrared spectroscopy reveals multi-step multi-timescale photoactivation in the photoconvertible protein archetype dronpa.
Article in Nature chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 64 citations in OpenAlex.
- Characterization of Parameter Uncertainty in Global Analysis for Ultrafast Spectroscopy Using Markov Chain Monte Carlo Sampling.Precision chemistry · 2026Article
- Ultrafast spectroscopic signatures forChemical science · 2026Article
- Dynamics and Mechanism of Off- to On-Switching in Dreiklang a Decoupled Reversibly Switchable Fluorescent Protein.Angewandte Chemie (International ed. in English) · 2026Article
- From Light to Life: Molecular Mechanisms and Macroscopic Transformations in Photoresponsive Hydrogels.Polymer science & technology (Washington, D.C.) · 2025Review
- All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.Nature communications · 2025Article
- Ultrafast photophysics of a positive reversibly switchable fluorescent protein.Chemical science · 2025Article
- Kinetic isotope effect reveals rate-limiting step in green-to-red photoconvertible fluorescent proteins.Protein science : a publication of the Protein Society · 2024Article
- Optical control of ultrafast structural dynamics in a fluorescent protein.Nature chemistry · 2023Article
- Breaking Barriers in Ultrafast Spectroscopy and Imaging Using 100 kHz Amplified Yb-Laser Systems.Accounts of chemical research · 2023Article
- Structural Characterization of Fluorescent Proteins Using Tunable Femtosecond Stimulated Raman Spectroscopy.International journal of molecular sciences · 2023Article
- Photogrammetry of Ultrafast Excited-State Intramolecular Proton Transfer Pathways in the Fungal Pigment Draconin Red.Molecules (Basel, Switzerland) · 2023Article
- Photophysics of the red-form Kaede chromophore.Chemical science · 2023Article
- Structural water molecules dominated p band intermediate states as a unified model for the origin on the photoluminescence emission of noble metal nanoclusters: from monolayer protected clusters to cage confined nanoclusters.Science and technology of advanced materials · 2023Review
- Observation of Cation Chromophore Photoisomerization of a Fluorescent Protein Using Millisecond Synchrotron Serial Crystallography and Infrared Vibrational and Visible Spectroscopy.The journal of physical chemistry. B · 2022Article
- Direct structural observation of ultrafast photoisomerization dynamics in sinapate esters.Communications chemistry · 2022Article
- Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.International journal of molecular sciences · 2022Review
- Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine.Nature communications · 2021Article
- Switching between Ultrafast Pathways Enables a Green-Red Emission Ratiometric Fluorescent-Protein-Based CaInternational journal of molecular sciences · 2021Article
- Picosecond to millisecond tracking of a photocatalytic decarboxylation reaction provides direct mechanistic insights.Nature communications · 2019Article
- Iminothioindoxyl as a molecular photoswitch with 100 nm band separation in the visible range.Nature communications · 2019Article
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 6 countries.
Funding
Abstract
Photochromic fluorescent proteins play key roles in super-resolution microscopy and optogenetics. The light-driven structural changes that modulate the fluorescence involve both trans-to-cis isomerization and proton transfer. The mechanism, timescale and relative contribution of chromophore and protein dynamics are currently not well understood. Here, the mechanism of off-to-on-state switching in dronpa is studied using femtosecond-to-millisecond time-resolved infrared spectroscopy and isotope labelling. Chromophore and protein dynamics are shown to occur on multiple timescales, from picoseconds to hundreds of microseconds. Following excitation of the trans chromophore, a ground-state primary product is formed within picoseconds. Surprisingly, the characteristic vibrational spectrum of the neutral cis isomer appears only after several tens of nanoseconds. Further fluctuations in protein structure around the neutral cis chromophore are required to form a new intermediate, which promotes the final proton-transfer reaction. These data illustrate the interplay between chromophore dynamics and the protein environment underlying fluorescent protein photochromism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.