ArticleViruses2018
The Odd "RB" Phage-Identification of Arabinosylation as a New Epigenetic Modification of DNA in T4-Like Phage RB69.
Article in Viruses, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 44 citations in OpenAlex.
- Dual cytosine modification through hydroxylation and arabinofuranosylation in phages.Nature chemical biology · 2026Article
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- END nucleases are antiphage defence systems targeting multiple phages with modified genomes.Nature microbiology · 2026Article
- DNA modifications of Durham Collection phages and promiscuity of GmrSD-family Type IV restriction enzyme BrxU.Applied and environmental microbiology · 2026Article
- Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.Nucleic acids research · 2026Article
- DNA-intercalating antiphage molecules trigger abortive infection through mutual destruction and synergize with bacterial immunity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A pair of DNA glucosyltransferases elevate counter-defense in bacteriophage T4.Nucleic acids research · 2026Article
- A pentose, as a cytosine nucleobase modification inApplied and environmental microbiology · 2026Article
- A LyticInternational journal of molecular sciences · 2026Article
- KlebPhaCol: a community-driven resource for Klebsiella research identified a novel phage family.Nucleic acids research · 2025Article
- Phages use contingency loci as a bet-hedging strategy.bioRxiv : the preprint server for biology · 2025Article
- Novel bacteriophages targeting wheat phyllosphere bacteria carry DNA modifications and single-strand breaks.Virus research · 2025Article
- AJournal of bacteriology · 2024Article
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- Temporal epigenome modulation enables efficient bacteriophage engineering and functional analysis of phage DNA modifications.PLoS genetics · 2024Article
- Phages carry orphan antitoxin-like enzymes to neutralize the DarTG1 toxin-antitoxin defense system.bioRxiv : the preprint server for biology · 2024Article
- Characterization of bacteriophages infecting multidrug-resistant uropathogenic Escherichia coli strains.Archives of virology · 2024Article
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- AbioRxiv : the preprint server for biology · 2024Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
In bacteriophages related to T4, hydroxymethylcytosine (hmC) is incorporated into the genomic DNA during DNA replication and is then further modified to glucosyl-hmC by phage-encoded glucosyltransferases. Previous studies have shown that RB69 shares a core set of genes with T4 and relatives. However, unlike the other “RB” phages, RB69 is unable to recombine its DNA with T4 or with the other “RB” isolates. In addition, despite having homologs to the T4 enzymes used to synthesize hmC, RB69 has no identified homolog to known glucosyltransferase genes. In this study we sought to understand the basis for RB69’s behavior using high-pH anion exchange chromatography (HPAEC) and mass spectrometry. Our analyses identified a novel phage epigenetic DNA sugar modification in RB69 DNA, which we have designated arabinosyl-hmC (ara-hmC). We sought a putative glucosyltranserase responsible for this novel modification and determined that RB69 also has a novel transferase gene, ORF003c, that is likely responsible for the arabinosyl-specific modification. We propose that ara-hmC was responsible for RB69 being unable to participate in genetic exchange with other hmC-containing T-even phages, and for its described incipient speciation. The RB69 ara-hmC also likely protects its DNA from some anti-phage type-IV restriction endonucleases. Several T4-related phages, such as
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