ArticleMolecules (Basel, Switzerland)2018
Quantification of Glutamate and Aspartate by Ultra-High Performance Liquid Chromatography.
Article in Molecules (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Article
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- Determination of Multiple Neurotransmitters through LC-MS/MS to Confirm the Therapeutic Effects ofMolecules (Basel, Switzerland) · 2023Article
- Metabolomic Approach to Study the 'Purple Queen' Pomegranate Cultivar Response to Alternative Culture Media and Phenological Stages.Foods (Basel, Switzerland) · 2023Article
- Identification of a novelFrontiers in genetics · 2022Article
- Article
- Variation for Composition and Quality in a Collection of the Resilient Mediterranean 'de penjar' Long Shelf-Life Tomato Under High and Low N Fertilization Levels.Frontiers in plant science · 2021Article
- Chemico-Biological Characterization of Torpedino Di FondiAntioxidants (Basel, Switzerland) · 2020Article
- GC-MS Based Metabolite Profiling to Monitor Ripening-Specific Metabolites in Pineapple (Metabolites · 2020Article
- Determination of the [Molecules (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glutamic and aspartic acid fulfil numerous functions in organisms. They are proteinogenic amino acids, they function as neurotransmitters, and glutamic acid links the citrate cycle with amino acid metabolism. In addition, glutamic acid is a precursor for many bioactive molecules like γ-aminobutyric acid (GABA). In tomatoes, glutamic acid accumulates in ripening fruits. Here we present a simple and rapid method for quantification of glutamate and aspartate in tomatoes. A cleared extract is prepared and 2-aminoadipic acid added as internal standard. Subsequently, the amino acids are derivatised with 2,4-dinitro-1-fluorobenzene under alkaline conditions. The derivatives are separated by ultra-high performance liquid chromatography using a phenyl-hexyl column and 50 mM
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.