ArticlePlastic and reconstructive surgery2018
A Simple Dynamic Strategy to Deliver Stem Cells to Decellularized Nerve Allografts.
Article in Plastic and reconstructive surgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Advancing Peripheral Nerve Regeneration (Nerve SPACE 2025).Journal of hand surgery global online · 2026Article
- Decellularized Biohybrid Nerve Promotes Motor Axon Projections.Advanced healthcare materials · 2024Article
- The Effect of Local Purified Exosome Product, Stem Cells, and Tacrolimus on Neurite Extension.The Journal of hand surgery · 2024Article
- Functional outcomes of nerve allografts augmented with mesenchymal stem cells and surgical angiogenesis in a rat sciatic nerve defect model.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2023Article
- Neuro-regenerative behavior of adipose-derived stem cells in aligned collagen I hydrogels.Materials today. Bio · 2023Article
- Safety of Allogeneic Mesenchymal Stem Cell Seeding of NeuraGen Nerve Guides in a Rabbit Model.Tissue engineering. Part C, Methods · 2023Article
- Role of adipose tissue grafting and adipose-derived stem cells in peripheral nerve surgery.Neural regeneration research · 2022Review
- Dynamic seeding versus microinjection of mesenchymal stem cells for acellular nerve allograft: an in vitro comparison.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2022Article
- Neural tissue engineering: From bioactive scaffolds and in situ monitoring to regeneration.Exploration (Beijing, China) · 2022Article
- Augmenting Peripheral Nerve Regeneration with Adipose-Derived Stem Cells.Stem cell reviews and reports · 2022Review
- Mesenchymal stem cells and local tacrolimus delivery synergistically enhance neurite extension.Biotechnology and bioengineering · 2021Article
- Harnessing 3D collagen hydrogel-directed conversion of human GMSCs into SCP-like cells to generate functionalized nerve conduits.NPJ Regenerative medicine · 2021Article
- Gene expression profiles of human adipose-derived mesenchymal stem cells dynamically seeded on clinically available processed nerve allografts and collagen nerve guides.Neural regeneration research · 2021Article
- Microcomputed analysis of nerve angioarchitecture after combined stem cell delivery and surgical angiogenesis to nerve allograft.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2021Article
- The interaction of stem cells and vascularity in peripheral nerve regeneration.Neural regeneration research · 2021Article
- Introducing human adipose-derived mesenchymal stem cells to AvanceJournal of plastic, reconstructive & aesthetic surgery : JPRAS · 2020Article
- Adipose derived mesenchymal stem cells seeded onto a decellularized nerve allograft enhances angiogenesis in a rat sciatic nerve defect model.Microsurgery · 2020Article
- Advances in the repair of segmental nerve injuries and trends in reconstruction.Muscle & nerve · 2020Review
- Adhesion, distribution, and migration of differentiated and undifferentiated mesenchymal stem cells (MSCs) seeded on nerve allografts.Journal of plastic, reconstructive & aesthetic surgery : JPRAS · 2020Article
- Gene expression and growth factor analysis in early nerve regeneration following segmental nerve defect reconstruction with a mesenchymal stromal cell-enhanced decellularized nerve allograft.Plastic and reconstructive surgery. Global open · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe addition of adipose-derived mesenchymal stromal cells to decellularized nerve allografts may improve outcomes of nerve reconstruction. Prior techniques used for cell seeding are traumatic to both the mesenchymal stromal cells and nerve graft. An adequate, reliable, and validated cell seeding technique is an essential step for evaluating the translational utility of mesenchymal stromal cell-enhanced decellularized nerve grafts. The purpose of this study was to develop a simple seeding strategy with an optimal seeding duration.
methodsA dynamic bioreactor was used to seed rat and human mesenchymal stromal cells separately onto rat and human decellularized nerve allografts. Cell viability was evaluated by MTS assays and cellular topology after seeding was determined by scanning electron microscopy. Cell density and distribution were determined by Live/Dead assays and Hoechst staining at four different time points (6, 12, 24, and 72 hours). The validity and reliability of the seeding method were calculated.
resultsCells remained viable at all time points, and mesenchymal stromal cells exhibited exponential growth in the first 12 hours of seeding. Seeding efficiency increased significantly from 79.5 percent at 6 hours to 89.2 percent after 12 hours of seeding (p = 0.004). Both intrarater reliability (r = 0.97) and interrater reliability (r = 0.92) of the technique were high.
conclusionsThis study describes and validates a new method of effectively seeding decellularized nerve allografts with mesenchymal stromal cells. This method is reproducible, distributes cells homogenously over the graft, and does not traumatize the intraneural architecture of the allograft. Use of this validated seeding technique will permit critical comparison of graft outcomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.