ArticleNature biotechnology2018
Precise, automated control of conditions for high-throughput growth of yeast and bacteria with eVOLVER.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
108 citing papers in PubMed.
- Article
- Engineered bacterial siderophore production accelerates rock weathering for carbon removal.Nature biotechnology · 2026Article
- Exploring the Evolutionary Landscape with Targeted In Vivo Hypermutations.Biomedicines · 2026Review
- OpenEvo: An Open-Source Platform for Automated Evolution and Analysis.bioRxiv : the preprint server for biology · 2026Article
- Physiological architecture and evolutionary origins of cellular adaptability.bioRxiv : the preprint server for biology · 2026Article
- Using TraDIS as a complementary approach to long term evolution for mapping adaptive mutations in Escherichia coli.PLoS genetics · 2026Article
- Lactic Acid Bacteria for Fungal Control and Shelf-Life Extension in Fresh Pasta: Mechanistic Insights and Clean-Label Strategies.Molecules (Basel, Switzerland) · 2026Review
- Article
- Linezolid and levofloxacin: an uncommon pairing that suppresses evolution of resistance inFEMS microbes · 2026Article
- Article
- Protein structure shapes natural genetic variation andbioRxiv : the preprint server for biology · 2025Article
- Advances in adaptive laboratory evolution applications forSynthetic and systems biotechnology · 2025Review
- Automated T-Cell Proliferation in Lab-on-Chip Devices Integrating Microfluidics and Deep Learning-Based Image Analysis for Long-Term Experiments.Biosensors · 2025Article
- BIO-SPEC: An open-source bench-top parallel bioreactor system.HardwareX · 2025Article
- The Yeast Optogenetic Toolkit (yOTK) for Spatiotemporal Control of Gene Expression in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Investigating the effects of liquid handling robot pipetting speed on yeast growth and gene expression using growth assays and RNA-seq.microPublication biology · 2025Article
- Highly parallelized laboratory evolution of wine yeasts for enhanced metabolic phenotypes.Molecular systems biology · 2024Article
- Development of specialized devices for microbial experimental evolution.Development, growth & differentiation · 2024Review
- Mapping the dynamics of epigenetic adaptation in S. pombe during heterochromatin misregulation.Developmental cell · 2024Article
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Precise control over microbial cell growth conditions could enable detection of minute phenotypic changes, which would improve our understanding of how genotypes are shaped by adaptive selection. Although automated cell-culture systems such as bioreactors offer strict control over liquid culture conditions, they often do not scale to high-throughput or require cumbersome redesign to alter growth conditions. We report the design and validation of eVOLVER, a scalable do-it-yourself (DIY) framework, which can be configured to carry out high-throughput growth experiments in molecular evolution, systems biology, and microbiology. High-throughput evolution of yeast populations grown at different densities reveals that eVOLVER can be applied to characterize adaptive niches. Growth selection on a genome-wide yeast knockout library, using temperatures varied over different timescales, finds strains sensitive to temperature changes or frequency of temperature change. Inspired by large-scale integration of electronics and microfluidics, we also demonstrate millifluidic multiplexing modules that enable multiplexed media routing, cleaning, vial-to-vial transfers and automated yeast mating.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.