ArticleJournal of visualized experiments : JoVE2018
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Size-independent and automated single-colony-resolution microdroplet dispensing.Lab on a chip · 2025Article
- Clinical application of single-cell RNA sequencing in disease and therapy.Clinical and translational medicine · 2025Review
- NOVAsort for error-free droplet microfluidics.Nature communications · 2024Article
- Innovative aspects and applications of single cell technology for different diseases.American journal of cancer research · 2024Review
- Recent progress and applications of single-cell sequencing technology in breast cancer.Frontiers in genetics · 2024Review
- Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.Science advances · 2023Article
- COMicromachines · 2022Article
- Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Going with the Flow: Modeling the Tumor Microenvironment Using Microfluidic Technology.Cancers · 2021Review
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Single-Cell Genomics: Enabling the Functional Elucidation of Infectious Diseases in Multi-Cell Genomes.Pathogens (Basel, Switzerland) · 2021Review
- The future of microfluidics in immune checkpoint blockade.Cancer gene therapy · 2021Review
- Crosslinking Strategies for the Microfluidic Production of Microgels.Molecules (Basel, Switzerland) · 2021Review
- Networks of High Aspect Ratio Particles to Direct Colloidal Assembly Dynamics and Cellular Interactions.Advanced functional materials · 2020Article
- Targeted Single-Cell RNA and DNA Sequencing With Fluorescence-Activated Droplet Merger.Analytical chemistry · 2020Article
- Single-Cell Sequencing of T cell Receptors: A Perspective on the Technological Development and Translational Application.Advances in experimental medicine and biology · 2020Review
- Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.Scientific reports · 2019Article
- Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology.Micromachines · 2019Review
- Engineered microscale hydrogels for drug delivery, cell therapy, and sequencing.Biomedical microdevices · 2019Review
- The single-cell sequencing: new developments and medical applications.Cell & bioscience · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Sequencing technologies have undergone a paradigm shift from bulk to single-cell resolution in response to an evolving understanding of the role of cellular heterogeneity in biological systems. However, single-cell sequencing of large populations has been hampered by limitations in processing genomes for sequencing. In this paper, we describe a method for single-cell genome sequencing (SiC-seq) which uses droplet microfluidics to isolate, amplify, and barcode the genomes of single cells. Cell encapsulation in microgels allows the compartmentalized purification and tagmentation of DNA, while a microfluidic merger efficiently pairs each genome with a unique single-cell oligonucleotide barcode, allowing >50,000 single cells to be sequenced per run. The sequencing data is demultiplexed by barcode, generating groups of reads originating from single cells. As a high-throughput and low-bias method of single-cell sequencing, SiC-seq will enable a broader range of genomic studies targeted at diverse cell populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.