ArticleJournal of visualized experiments : JoVE2018
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum.
Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Article
- Sja-let-7 suppresses the development of liver fibrosis via Schistosoma japonicum extracellular vesicles.PLoS pathogens · 2024Article
- Molecular and functional characterization of Schistosoma japonicum annexin A13.Veterinary research · 2023Article
- Guidelines for the purification and characterization of extracellular vesicles of parasites.Journal of extracellular biology · 2023Article
- Exosomes Derived from BMMSCs Mitigate the Hepatic Fibrosis via Anti-Pyroptosis Pathway in a Cirrhosis Model.Cells · 2022Article
- Proteomic Analysis of Plasma-Derived Extracellular Vesicles From Mice WithFrontiers in cellular and infection microbiology · 2022Article
- Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon.Journal of nanobiotechnology · 2021Article
- Exosomal microRNA let-7-5p fromMicroorganisms · 2021Article
- Proteomic and deep sequencing analysis of extracellular vesicles isolated from adult male and female Schistosoma japonicum.PLoS neglected tropical diseases · 2020Article
- Schistosoma japonicum extracellular vesicle miRNA cargo regulates host macrophage functions facilitating parasitism.PLoS pathogens · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) are membranous vesicles released by a variety of cells into the extracellular microenvironment. EVs represent a population of heterogeneous vesicles, whose size range between 40 and 1,000 nm. Accumulated evidence indicated that EVs play important regulatory roles in pathogen-host interactions. A deep understanding of schistosome EVs should provide insights into the mechanisms underlying schistosome-host interactions, enabling development of novel strategies against schistosomiasis. Here, we aim to further study EVs functions in schistosomes by presenting a protocol for the isolation and characterization of EVs from adult Schistosoma japonicum (S. japonicum). EVs were isolated from in vitro culture medium using centrifugation combined with a commercial exosome isolation kit. The isolated S. japonicum EVs (SjEVs) typically possess a diameter of 100 - 400 nm, and are characterized by transmission electronic microscopy and western blotting. The usage of PKH67 dye-labeled SjEVs has demonstrated that SjEVs are internalized by the recipient cells. Overall, our protocol provides an alternative method for isolating EVs from adult schistosomes; the isolated SjEVs may be suitable for functional analysis.
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