Evidence map›Paper›PMID 29889187›Full record

ArticleJournal of visualized experiments : JoVE2018

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection.

Hye-Young Lee, So Eui Lee, Jongchan Woo, Doil Choi, Eunsook Park

Open access · bronzeAbstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Hye-Young LeeDepartment of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University.
So Eui LeeDepartment of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University.
Jongchan WooDepartment of Bioindustry and Bioresource Engineering, Sejong University.
Doil ChoiDepartment of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University; doil@snu.ac.kr.
Eunsook ParkDepartment of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University; arabidopsis1004@snu.ac.kr.
Seoul National University · KRSejong University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacteria, one of the most important causative agents of various plant diseases, secrete a set of effector proteins into the host plant cell to subvert the plant immune system. During infection cytoplasmic effectors are delivered to the host cytosol via a type III secretion system (T3SS). After delivery into the plant cell, the effector(s) targets the specific compartment(s) to modulate host cell processes for survival and replication of the pathogen. Although there has been some research on the subcellular localization of effector proteins in the host cells to understand their function in pathogenicity by using fluorescent proteins, investigation of the dynamics of effectors directly injected from bacteria has been challenging due to the incompatibility between the T3SS and fluorescent proteins. Here, we describe our recent method of an optimized split superfolder green fluorescent protein system (sfGFP

Indexed as

ArabidopsisBacterial ProteinsGreen Fluorescent ProteinsPlant DiseasesPseudomonas syringaeBacterial ProteinsGreen Fluorescent Proteins

Identifiers

PMID29889187
PMCPMC6101373
OpenAlexW2803662678

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.