ArticleAmerican journal of translational research2018
Synergistic effect of a LPEMF and SPIONs on BMMSC proliferation, directional migration, and osteoblastogenesis.
Article in American journal of translational research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of Low-Frequency Electromagnetic Fields on Mesenchymal Stem Cells Differentiation: A Systematic Review.Tissue engineering and regenerative medicine · 2022Pooled it
- Review
- Biophysical control of plasticity and patterning in regeneration and cancer.Cellular and molecular life sciences : CMLS · 2023Review
- Overview of Antimicrobial Biodegradable Polyester-Based Formulations.International journal of molecular sciences · 2023Review
- Pulsed Electromagnetic Field Therapy and Direct Current Electric Field Modulation Promote the Migration of Fibroblast-like Synoviocytes to Accelerate Cartilage Repair In Vitro.Applied sciences (Basel, Switzerland) · 2022Article
- Exposure of primary osteoblasts to combined magnetic and electric fields induced spatiotemporal endochondral ossification characteristic gene- and protein expression profiles.Journal of experimental orthopaedics · 2022Article
- Hope for bone regeneration: The versatility of iron oxide nanoparticles.Frontiers in bioengineering and biotechnology · 2022Review
- Extracellular Vesicles in Bone Homeostasis: Emerging Mediators of Osteoimmune Interactions and Promising Therapeutic Targets.International journal of biological sciences · 2022Review
- Pulsed Electromagnetic Fields in Bone Healing: Molecular Pathways and Clinical Applications.International journal of molecular sciences · 2021Review
- Magnetic ion channel activation of TREK1 in human mesenchymal stem cells using nanoparticles promotes osteogenesis in surrounding cells.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pulsed electromagnetic fields (PEMFs) represent a new type of physiotherapy that has been shown to be effective for improving bone fracture healing and treating osteoporosis. Targeted therapy with bone marrow mesenchymal stem cells (BMMSCs) has been the focus of several recent studies. The key to such therapy is the effective application of certain nanomaterials in BMMSCs so they achieve an ideal target concentration under the influence of a PEMF. In our present study, the effects of a PEMF on the process of osteoblastogenesis were systematically investigated using superparamagnetic iron oxide nanoparticle (SPION)-labeled BMMSCs. Rat BMMSCs labeled with SPIONs were exposed to a low-frequency pulsed electromagnetic field (LPEMF) of 50 Hz at 1.1 mT. Exposure to the LPEMF resulted in an enhanced proliferation of SPION-labeled BMMSCs when compared with a control group. Furthermore, observations made by transmission electron microscopy (TEM) revealed greater cell concentrations in the central zone with exposure to the LPEMF than in the peripheral zone without LPEMF stimulation, indicating that a LPEMF could induce the migration of SPION-labeled BMMSCs towards a magnetic field. Transwell experiments confirmed that combining SPIONs with a LPEMF could significantly promote the directional migration of BMMSCs. Von Kossa and ALP staining of LPEMF-exposed SPION-labeled cells was more intense, and those cells displayed higher levels of ALP activity than control cells. The SPION-labeled, LPEMF-exposed cells also showed increased levels of osteogenesis-related gene and protein expression (e.g., ALP, OCN, and RUNX2) in PCR and western blot studies. Taken together, our findings suggest that a combination of LPEMF and SPIONs exerts a synergistic effect on promoting the directional migration and osteogenic differentiation of BMMSCs, indicating that application of a LPEMF in conjunction with SPIONs may constitute a method for treating bone defects.
Indexed as
Identifiers
29887957PMC5992538What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.