ArticleStem cell reports2018
Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neural Connections with Mouse Central Auditory Neurons In Vitro via the α2δ1 Receptor.
Article in Stem cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Recent Advances in Mechanobiology-Mediated In Situ Tissue Engineering.International journal of biomaterials · 2026Review
- Article
- Neural stem cells in adult neurogenesis and their therapeutic applications in neurodegenerative disorders: a concise review.Frontiers in molecular medicine · 2025Review
- Inner Ear Drug Delivery for Sensorineural Hearing Loss: Current Challenges and Opportunities.Frontiers in neuroscience · 2022Review
- Postnatal Changes of Neural Stem Cells in the Mammalian Auditory Cortex.International journal of molecular sciences · 2021Article
- Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells.Tissue engineering. Part A · 2021Article
- Cochlear Sox2Frontiers in cell and developmental biology · 2021Article
- Electrical Stimulation Promotes Stem Cell Neural Differentiation in Tissue Engineering.Stem cells international · 2021Review
- Generation of a Spiral Ganglion Neuron Degeneration Mouse Model.Frontiers in cell and developmental biology · 2021Article
- Thrombospondin-1 in maladaptive aging responses: a concept whose time has come.American journal of physiology. Cell physiology · 2020Review
- Stem Cell-Based Therapeutic Approaches to Restore Sensorineural Hearing Loss in Mammals.Neural plasticity · 2020Review
- Identification of Neural Stem Cells from Postnatal Mouse Auditory Cortex In Vitro.Stem cells and development · 2019Article
- Generation of new hair cells by DNA methyltransferase (Dnmt) inhibitor 5-azacytidine in a chemically-deafened mouse model.Scientific reports · 2019Article
- [Research progress on the role of thrombospondin in synapse formation].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2019Review
- Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices.Stem cells international · 2019Article
- Optogenetically modified human embryonic stem cell-derived otic neurons establish functional synaptic connection with cochlear nuclei.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Integration of stem cell-derived neurons into the central nervous system (CNS) remains a challenge. A co-culture system is developed to understand whether mouse embryonic stem cell (ESC)-derived spiral ganglion neuron (SGN)-like cells (ESNs) synapse with native mouse cochlear nucleus (CN) neurons. A Cre system is used to generate Cop-GFP ESCs, which are induced into ESNs expressing features similar to auditory SGNs. Neural connections are observed between ESNs and CN neurons 4-6 days after co-culturing, which is stimulated by thrombospondin-1 (TSP1). Antagonist and loss-of-function small hairpin RNA studies indicate that the α2δ1 receptor is critical for TSP1-induced synaptogenesis effects. Newly generated synapse-like structures express pre- and post-synaptic proteins. Synaptic vesicle recycling, pair recording, and blocker electrophysiology suggest functional synaptic vesicles, transsynaptic activities, and formation of glutamatergic synapses. These results demonstrate the synaptogenesis capability of ESNs, which is important for pluripotent ESC-derived neurons to form functional synaptic connections to CNS neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.