Evidence map›Paper›PMID 29886541›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Study of Amyloids Using Yeast.

Reed B Wickner, Dmitry Kryndushkin, Frank Shewmaker, Ryan McGlinchey, Herman K Edskes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Antiprion systems in yeast cooperate to cure or prevent the generation of nearly all [Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  5. Nonsense Mutations in the YeastInternational journal of molecular sciences · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Reed B WicknerLaboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. wickner@helix.nih.gov.
Dmitry KryndushkinLaboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Frank ShewmakerDepartment of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.
Ryan McGlincheyLaboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Herman K EdskesLaboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.

Funding

Prions of Yeast and Anti-Prion SystemsZIADK024950 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI WICKNER, REED B. · 2009 to 2025
$25.2M
Using naturally-occurring fungal prions as models of neurodegenerative protein misfoldingR35GM119790 · NIGMS · HENRY M. JACKSON FDN FOR THE ADV MIL/MED · PI FENG, YUANYI · 2016 to 2020
$1.4M
PRIONS OF YEASTZ01DK024943 · NIDDK · DIABETES, DIGESTIVE, KIDNEY DISEASES · PI WICKNER, REED B. · 1994 to 2006
–
Intramural NIH HHS Z01 DK024943NIGMS NIH HHS R35 GM119790
6 · The paper itself

Abstract

We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.

Indexed as

Green Fluorescent ProteinsMicroscopy, Electron, TransmissionMutationPrion ProteinsRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsGreen Fluorescent ProteinsPrion ProteinsRecombinant ProteinsSaccharomyces cerevisiae ProteinsCytoductionElectron diffractionMass per lengthProtein transformation

Identifiers

PMID29886541
PMCPMC7337124

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.