Evidence map›Paper›PMID 29885806›Full record

ArticleVeterinary microbiology2018

Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing.

Janine T Bossé, Yanwen Li, Roberto Fernandez Crespo, Sonia Lacouture, Marcelo Gottschalk, Rita Sárközi, László Fodor, Maria Casas Amoribieta, Øystein Angen, Katerina Nedbalcova and 7 more

Abstract readComparative Study
In one paragraph

Article in Veterinary microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed.

  1. Article
  2. Review
  3. Journal of veterinary science · 2026
    Article
  4. Article
  5. Article
  6. Serotyping ofMicrobial genomics · 2025
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Characterization of nonencapsulatedJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Microbial genomics · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Janine T BosséSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK. Electronic address: j.bosse@imperial.ac.uk.
Yanwen LiSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK.
Roberto Fernandez CrespoSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK.
Sonia LacoutureGroupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Québec, Canada.
Marcelo GottschalkGroupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Québec, Canada.
Rita SárköziDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
László FodorDepartment of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
Maria Casas AmoribietaOVISLAB S.L., Barcelona, Spain.
Øystein AngenDepartment of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.
Katerina NedbalcovaVeterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Matthew T G HoldenThe Wellcome Trust Sanger Institute, Hinxton, UK.
Duncan J MaskellDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Alexander W TuckerDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Brendan W WrenFaculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
Andrew N RycroftDepartment of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, UK.
Paul R LangfordSection of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, UK.
BRaDP1T consortium

Funding

Biotechnology and Biological Sciences Research Council BB/G018553/1Biotechnology and Biological Sciences Research Council BB/G019177/1Biotechnology and Biological Sciences Research Council BB/G019274/1Biotechnology and Biological Sciences Research Council BB/G020744/1Wellcome Trust 098051
6 · The paper itself

Abstract

Problems with serological cross-reactivity have led to development of a number of PCRs (individual and multiplex) for molecular typing of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia. Most of these assays were developed for detection of specific amplicons within capsule biosynthetic genes before the availability of complete sequences for the different serovars. Here we describe comparative analysis of the complete capsular loci for all 18 serovars of A. pleuropneumoniae, and development of two multiplex PCRs for comprehensive capsule typing of this important pig pathogen.

Indexed as

Sequence AnalysisActinobacillus InfectionsActinobacillus pleuropneumoniaeAnimalsBacterial CapsulesMultiplex Polymerase Chain ReactionPolysaccharides, BacterialSerogroupSerotypingSwineSwine DiseasesPolysaccharides, BacterialA. pleuropneumoniaeCapsule typingDiagnosticmPCRSerovars

Identifiers

PMID29885806
PMCPMC6008488

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.