Evidence map›Paper›PMID 29885798›Full record

ArticleVeterinary microbiology2018

Bovine respiratory syncytial virus infection enhances Pasteurella multocida adherence on respiratory epithelial cells.

Putu Eka Sudaryatma, Kimika Nakamura, Hirohisa Mekata, Satoshi Sekiguchi, Meiko Kubo, Ikuo Kobayashi, Mawar Subangkit, Yoshitaka Goto, Tamaki Okabayashi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
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  4. Article
  5. Review
  6. Review
  7. Article
  8. Article
  9. Article
  10. Microorganisms · 2022
    Article
  11. Article
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  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. The Immunology of Bovine Respiratory Disease: Recent Advancements.The Veterinary clinics of North America. Food animal practice · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Putu Eka SudaryatmaGraduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Kimika NakamuraDepartment of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Hirohisa MekataCenter for Animal Disease Control, University of Miyazaki, Miyazaki, Japan; Organization for Promotion of Tenure Track University of Miyazaki, Miyazaki, Japan.
Satoshi SekiguchiDepartment of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Meiko KuboMiyakonojo Meat Inspection Center Miyazaki Prefecture Government, Miyazaki, Japan.
Ikuo KobayashiCenter for Animal Disease Control, University of Miyazaki, Miyazaki, Japan; Sumiyoshi education farm, University of Miyazaki, Miyazaki, Japan.
Mawar SubangkitGraduate School of Medicine and Veterinary Medicine, University of Miyazaki, Miyazaki, Japan; Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Yoshitaka GotoDepartment of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Tamaki OkabayashiDepartment of Veterinary Science, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan. Electronic address: okbys81@cc.miyazaki-u.ac.jp.
University of Miyazaki · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primary infection with bovine respiratory syncytial virus (BRSV) predisposes cattle to secondary infection with bacteria that cause bovine respiratory disease complex (BRDC). However, the interaction between BRSV and bacteria is unclear. This in vitro study examined the adherence of Pasteurella multocida (PM) to BRSV-infected cells was assessed in colony forming unit assays, by flow cytometry analysis, and by indirect immunofluorescence analysis (IFA) of epithelial cells (A549, HEp-2, and MDBK). An in vitro model based on infection of BRSV-infected epithelial cells revealed that PM adherence to BRSV-infected cells was 2- to 8-fold higher than uninfected cells. This was confirmed by flow cytometry analysis and IFA. Epithelial cell expression of mRNA encoding cytokines and chemokines increased after exposure to PM, but increased further after co-infection with BRSV and PM. BRSV-mediated adherence of PM to epithelial cells may underlie the serious symptoms of BRDC.

Indexed as

Microbial InteractionsA549 CellsAnimalsAttachment Sites, MicrobiologicalBovine Respiratory Disease ComplexCattleCattle DiseasesEpithelial CellsFlow CytometryFluorescent Antibody Technique, IndirectHumansPasteurella multocidaRespiratory Syncytial Virus, BovineRespiratory Syncytial Virus InfectionsRespiratory SystemAdherent bacteriaBRDCBRSVCo-infection

Identifiers

PMID29885798
PMCPMC7117154
OpenAlexW2799968185

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.